Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
Vol. 16, No. 2, 2017, pp. 421-427
Bioline Code: pr17055
Full paper language: English
Document type: Research Article
Document available free of charge
Tropical Journal of Pharmaceutical Research, Vol. 16, No. 2, 2017, pp. 421-427
© Copyright 2017 - Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria.
Identification of metabolites of gardenin A in rat liver microsomes using ultra-high performance liquid chromatography coupled with linear ion-trap Orbitrap mass spectrometry|
Cai, Rui Jun; Wei, Jie; Jing, Feng Ling; Zhao, Gui Zhen & Zhang, Yan
Purpose: To identify the metabolites of gardenin A (GA) in rat liver microsomes (RLMs) using ultra-high
performance liquid chromatography coupled with linear ion-trap Orbitrap mass spectrometry (UHPLC-LTQ-Orbitrap).
Methods: The sample was prepared by incubating GA (100 μg/mL) with RLMs (0.5 mg/mL) for 8 h.
Then 5 μL of the sample was injected into UHPLC-LTQ- orbitrap mass spectrometer. The metabolites of
GA were tentatively identified based on accurate mass measurements, fragmentation patterns,
chromatographic retention times, and bibliography data.
Results: A total of 12 metabolites were detected and identified. Based on their structures, the main
reactions in the metabolism of GA are de-methoxylation and de-methylation.
Conclusion: This is the first report on in vitro metabolites of GA. These results are considered very
helpful for better comprehension of the metabolism of GA and its pharmacological effects.
Gardenin A; Metabolites; UHPLC-LTQ-Orbitrap; Rat Liver microsomes
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