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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 16, No. 2, 2017, pp. 421-427
Bioline Code: pr17055
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 16, No. 2, 2017, pp. 421-427

 en Identification of metabolites of gardenin A in rat liver microsomes using ultra-high performance liquid chromatography coupled with linear ion-trap Orbitrap mass spectrometry
Cai, Rui Jun; Wei, Jie; Jing, Feng Ling; Zhao, Gui Zhen & Zhang, Yan

Abstract

Purpose: To identify the metabolites of gardenin A (GA) in rat liver microsomes (RLMs) using ultra-high performance liquid chromatography coupled with linear ion-trap Orbitrap mass spectrometry (UHPLC-LTQ-Orbitrap).
Methods: The sample was prepared by incubating GA (100 μg/mL) with RLMs (0.5 mg/mL) for 8 h. Then 5 μL of the sample was injected into UHPLC-LTQ- orbitrap mass spectrometer. The metabolites of GA were tentatively identified based on accurate mass measurements, fragmentation patterns, chromatographic retention times, and bibliography data.
Results: A total of 12 metabolites were detected and identified. Based on their structures, the main reactions in the metabolism of GA are de-methoxylation and de-methylation.
Conclusion: This is the first report on in vitro metabolites of GA. These results are considered very helpful for better comprehension of the metabolism of GA and its pharmacological effects.

Keywords
Gardenin A; Metabolites; UHPLC-LTQ-Orbitrap; Rat Liver microsomes

 
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