Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
Vol. 16, No. 3, 2017, pp. 525-533
Bioline Code: pr17068
Full paper language: English
Document type: Research Article
Document available free of charge
Tropical Journal of Pharmaceutical Research, Vol. 16, No. 3, 2017, pp. 525-533
© Copyright 2017 - Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria.
Elucidation of mechanism of Si-jun-zi decoction-induced reversal of spleen deficiency syndrome in rats by LC-Q-TOF/MS metabolomics|
Yan, Qinghua; Mao, Huili & Wei, Yanming
Purpose: To explore the therapeutic effect and mechanism of action of Si–jun–zi decoction (SJZD) in
rat spleen deficiency syndrome.
Methods: Spleen deficiency syndrome was induced in a rat model with reserpine. Liquid
chromatography quadrupole time-of-flight mass spectrometry (LC-Q-TOF/MS) and partial least squares-discriminate
models (PLS-DA) were used to analyze the difference in global metabolite profile within all
groups. Variable importance projection (VIP > 1) and Student’s t–test (p < 0.05) were used for
Results: Enzymatic results showed that the level of amylase (AMY), pepsin (LPS) and pepsase were
significantly lower in the untreated rats than in normal control rats and SJZD group rats (P < 0.05).
Histopathological analysis showed there were significant pathological changes in the untreated rats
compared with the normal control group and SJZD group rats. PLS-DA score plots of the untreated
group rats clustered separately from control rats, whereas SJZD group rats clustered closely to normal
control rats. Twenty potential biomarkers, including phospholipid and fatty acids, were screened. The
relative content of 20 metabolites showed significant differences in the untreated group compared with
control and SJZD groups (p < 0.05). Six potentially perturbed metabolic pathways were found, which
might be potential targets pathways for SJZD intervention in spleen deficiency syndrome.
Conclusion: Metabolomics approach is an effective tool for understanding the therapeutic mechanisms
Si–jun–zi decoction; Spleen deficiency syndrome; Metabolomics; LC-Q-TOF/MS
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