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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996 EISSN: 1596-5996
Vol. 16, No. 4, 2017, pp. 755-760
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Bioline Code: pr17096
Full paper language: English
Document type: Research Article
Document available free of charge
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Tropical Journal of Pharmaceutical Research, Vol. 16, No. 4, 2017, pp. 755-760
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Label-free biochips for rapid detection of soybean allergen GlymBd 30K (P34) in foods
Wang, Wei; Zhu, Xudong; Teng, Shuang; Fan, Qiaojun & Qian, Hang
Abstract
Purpose: To develop an innovative method for detection of soybean allergen, Gly mBd 30K (P34) in
foods using a biosensor based on high spatial imaging ellipsometer.
Methods: Two monoclonal antibodies, 2D1 and 5F9, each known to have specific bioactivity against
P34 allergen, were selected and separately immobilized as ligands on silicon wafer surface to allow
capture of the P34 allergen. The resultant changes on the wafer surface were viewed directly as images
in gray scale.
Results: Images indicated that these two antibodies detected the presence of P34 allergen in soybean
extract with sensitivity of 1 mg/L and a detection time of about 15 min. For the detection of P34 allergen
in foods, results from biochip detection were consistent with those obtained using ELISA detection.
Conclusion: These results show that the biochip may be an effective analytical tool for food allergen
detection.
Keywords
Soybean Allergen, Gly mBd 30K, Biochip, Detection, Foods
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