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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996 EISSN: 1596-5996
Vol. 16, No. 4, 2017, pp. 771-779
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Bioline Code: pr17098
Full paper language: English
Document type: Research Article
Document available free of charge
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Tropical Journal of Pharmaceutical Research, Vol. 16, No. 4, 2017, pp. 771-779
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MicroRNA and gene signature of severe cutaneous drug hypersensitivity reactions reveal the role of miR-483-5p/miR-28-5p in inflammation by targeting Granulysin gene
Elbakkoush, Abdallah Ahmed; Khaleel, Anas & Liu, Chien-Tsai
Abstract
Purpose: To build a microRNA and gene signature of severe cutaneous adverse drug reactions
(SCAR), including Stevens-Johnson syndrome (SJS) and toxic epidermal necrolysis (TEN).
Methods: MicroRNA expression profiles were downloaded from miRNA expression profile of patients’
skin suffering from TEN using an array comprising of 372 miRNAs; download site: www.jacionline.org.
The patient samples were eight TEN, ten SJS patients and twenty-two healthy individuals. A total of 192
microRNAs were found with unique expression patterns (overexpressed) in contrast with healthy skin
controls and patients. Thereafter, the following databases were used for downstream analysis:
geneMANIA, DIANA-miRPath version 3, DIANA-TarBase version 7.0, Ingenuity Pathway Analysis (IPA)
as well as DAVID, STRING and GENECODIS online tools.
Results: Granulysin (GNLY) geneMANIA database search yielded 21 interacting genes that were 64.6
% in physical interaction, 17 % in co-expression pattern. miRBD potential microRNAs that target the 21
genes were 79 miRs. Eighteen miRs overlap between the overexpressed miRs from SJS/TEN samples
and the miRs targeting the 21 genes. Moreover, Ingenuity pathway analysis IPA revealed that the
microRNAs were involved in inflammation.
Conclusion: Analysis of differential microRNA expressions reveals two significant DE miRs that target
Granulysin (483-5p/miR-28-5p). MiR-GNLY loop interactions in hypersensitivity reactions may function
as biomarkers for SCAR including SJS and TEN.
Keywords
Severe cutaneous adverse drug reactions (SCAR) Steven-Johnson Syndrome, Toxic epidermal necrolysis, Granulysin, Biomarkers, MicroRNA signature
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© Copyright 2017 - Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria. Alternative site location: http://www.tjpr.org
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