|
Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996 EISSN: 1596-5996
Vol. 16, No. 5, 2017, pp. 1129-1136
|
Bioline Code: pr17145
Full paper language: English
Document type: Research Article
Document available free of charge
|
|
Tropical Journal of Pharmaceutical Research, Vol. 16, No. 5, 2017, pp. 1129-1136
en |
Extraction optimization and characterization of polysaccharide antioxidants from Pinellia ternata (Thunb) Breit rhizome
Mo, Xuelin; Liu, Yujie; Li, Tao; Peng, Wei; Hu, Meibian & Wu, Chunjie
Abstract
Purpose: To investigate the optimum extraction conditions of polysaccharides from Pinellia Rhizoma
(PRP) and their antioxidant activities.
Methods: Response surface methodology (RSM) was applied to optimize the water extraction
conditions of PRP by Box-Benhnken design (BBD). A high performance liquid chromatography (HPLC)
method was performed for determining the monosaccharide composition, while a high performance gel
permeation chromatography (HPGPC) method was established for determining the molecular weight
distribution of PRP. In addition, scavenging DPPH and superoxide anion radical scavenging assays
were used to evaluate the antioxidant activities of PRP.
Results: As a result, the optimum extraction conditions of PRP were as follows: extraction time, 103
min; solid-liquid ratio, 24 g/mL; and extraction no., 3. Under these conditions, the maximum extraction
yield of PRP was 2.47 %, which matched the optimum value (2.55 %) predicted by RSM. The results of
HPLC analysis suggest that the monosaccharide composition of PRP was mannose (Man), galactose
acid (Gal acid), galactose (Gal), glucose (Glc), and arabinose (Ara) in molar ratios of
5.76:2.20:2.64:4.57:1, respectively, and the molecular weight ranged from 13592 to 445065.
Furthermore, PRP showed marked antioxidant activities with the highest DPPH free radical scavenging
rate of 71.5 % and O2- free radical scavenging rate of 87.5 % at a concentration of 10 mg/mL.
Conclusion: RSM is a rapid and effective statistical technique for optimizing conditions for PRP
extraction. Furthermore, PRP is a potential natural source of antioxidants.
Keywords
Pinellia rhizoma; Polysaccharides Optimization extraction; Monosaccharide composition; Antioxidant activity
|
|
© Copyright 2017 - Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria. Alternative site location: http://www.tjpr.org
|
|