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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996 EISSN: 1596-5996
Vol. 16, No. 8, 2017, pp. 1893-1901
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Bioline Code: pr17240
Full paper language: English
Document type: Research Article
Document available free of charge
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Tropical Journal of Pharmaceutical Research, Vol. 16, No. 8, 2017, pp. 1893-1901
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Mitigation of chronic unpredictable stress–induced cognitive deficits in mice by Lycium barbarum L (Solanaceae) polysaccharides
Xue, Min-Qiu; Quan, Hong-Feng; Wang, Rui; Zhao, Xun-Xia; Yan, Lin; Zhu, Ya-Fei; Jing, Jing; Hua, Bing & Peng, Xiao-Dong
Abstract
Purpose: To investigate the neuroprotective effects of Lycium barbarum polysaccharide (LBP) against
concomitant cognitive dysfunction and changes in hippocampal CREB-BDNF signaling pathway in
chronically unpredictable stressed mice.
Methods: The mice were subjected to different unpredictable stressors for a period of 4 weeks.
Behavioral tests, including open field (OFT) and Morris water maze (MWMT) tests were used to
evaluate pharmacological effects. Serum corticosterone levels, protein expression level of BDNF and
pCREB/CREB in hippocampus were assessed by ELISA, Western blot and immunohistochemistry
methods, respectively. Morphological changes in pyramidal neurons in the hippocampus were studied
by Nissl staining.
Results: LBP improved mice performance in MWMT, indicating that it reversed chronic unpredictable
stress (CUS)-induced cognitive deficits. LBP treatment reduced serum corticosterone levels and
prevented neuron loss in the hippocampus. It maintained expression levels of BDNF and
phosphorylation of CREB in hippocampus during CUS procedure.
Conclusion: Lycium barbarum polysaccharide protects CREB-BDNF signaling pathway in
hippocampus and relieves CUS-induced cognitive deficits. These results suggest that Lycium barbarum
polysaccharides is potentially an alternative neuro-protective agent against stress-induced
psychopathological dysfunction.
Keywords
Lycium barbarum; Polysaccharide; Chronic unpredictable stress; Cognitive deficits; Brain-derived neurotrophic factor; Calcium/cyclic-AMP responsive binding protein
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