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Iranian Journal of Pharmacology and Therapeutics
Razi Institute for Drug Research (RIDR) of Iran University of Medical Sciences and Health Services (IUMS)
ISSN: 1735-2657
Vol. 2, No. 1, 2003, pp. 18-21
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Bioline Code: pt03004
Full paper language: English
Document type: Research Article
Document available free of charge
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Iranian Journal of Pharmacology and Therapeutics, Vol. 2, No. 1, 2003, pp. 18-21
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A High-Performance Liquid Chromatographic Assay for the Determination of Losartan in Plasma
Hassan Jalalizadeh, Effat Souri, Hassan Farsam and Mehdi Ansari
Abstract
A rapid and sensitive HPLC method was developed for determination of losartan in plasma. Losartan was extracted from plasma by a two-step extraction procedure using chloroform as extracting solvent in acidic medium. HPLC analysis was performed on a cyano reversed-phase column using phosphate buffer (pH 4.3), acetonitrile (750:250, v/v) as mobile phase with a flow rate of 0.9 mL/min. Sodium diclofenac was selected as internal standard. Excellent linearity between the peak area ratios and losartan concentrations over the range of 2-200 ng/mL of plasma was observed. The limit of determination with UV detection at 225 nm, with a CV < 5% was 2 ng/mL in 500 μL of plasma sample. The assay was rapid, safe and reliable for use in pharmacokinetic studies of losartan in human being.
Keywords
Losartan, Plasma, Extraction, HPLC analysis
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© Copyright 2003 - Razi Institute for Drug Research (RIDR) Alternative site location: http://ijpt.iums.ac.ir/
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