search
for
 About Bioline  All Journals  Testimonials  Membership  News


Revista Colombia Médica
Universidad del Valle - Facultad de Salud
ISSN: 0120-8322
EISSN: 0120-8322
Vol. 38, No. 3, 2007, pp. 197-209
Bioline Code: rc07042
Full paper language: Spanish
Document type: Research Article
Document available free of charge

Revista Colombia Médica, Vol. 38, No. 3, 2007, pp. 197-209

 en Apoptosis of gingival fibroblasts in periodontitis
Arce, Roger Mauricio; Tamayo, Oscar & Cortés, Armando

Abstract

Introduction: Human gingival fibroblasts (HGF) have an important role in the periodontal immune response. The fibroblasts alter their normal behavior in response to pro-inflammatory cytokines. It is believed that HGF can be diminished and/or eliminated by means of apoptosis. The purpose of this study was to determine and to quantify apoptosis of HGF in periodontium biopsies from healthy and chronic periodontitis patients.
Methods: A clinical cross-sectional study in people with healthy periodontium (S), gingivitis (G) and chronic periodontitis (PC) patients was carried out. The periodontal biopsies were obtained and immunostained by means of: hematoxylin-eosin, caspase-3, vimentin and caspase-vimentin double-staining for specific visualization of apoptotic fibroblasts. Histopathological and digital analyses were performed. Descriptive statistics were applied to categorical and nominal variables.
Results: Total cell population of HGF had an average of 430±67.6 cells/field in healthy people, and a significantly progressive decrease in gingivitis (270±37.1) and chronic periodontitis groups (206.5±69.8) (p<0.05). As for total population of inflammatory cells, an increase was noticed in gingivitis (191.8±50.1) and a decrease in periodontitis (109.3±21.7) without statistical significance. The expression of apoptotic HGF per field increased accordingly to the severity of the disease [28±16 in health (6.5%); 31±17 in gingivitis (11.5%) and 51±24 in periodontitis (24.8%), p<0.001]. Similar findings were observed for inflammatory cells with different percentage expression [17±13 in health (23%); 28±19 in gingivitis (14.6%) and 47±35 in periodontitis (43.1%), p<0.05]. The relationship between the percentage of expression of apoptotic cells and probing pocket depth was proportional but not significant (p>0.5, r2=0.02); while for the inflammatory cells a significant relationship was observed (p<0.05, r2=0.2018).
Conclusions: The results in this report conclude that HGF and inflammatory cells show apoptosis (caspase-3 expression), and apoptotic cells are significantly increased in gingivitis and chronic periodontitis groups.

Keywords
Periodontitis; Gingivitis; Apoptosis; Pathogenesis; Gingival; Fibroblasts; Immunohistochemistry.

 
 es Apoptosis de fibroblastos gingivales en periodontitis
Arce, Roger Mauricio; Tamayo, Oscar & Cortés, Armando

Resumen

Introducción: Los fibroblastos gingivales humanos (FGH) tienen un papel importante en la enfermedad periodontal, pues alteran su normal funcionamiento en respuesta a estímulos pro-inflamatorios. Se cree que los fibroblastos se pueden eliminar anormalmente por medio de apoptosis en periodontitis. El propósito de este estudio es determinar y cuantificar la apoptosis de FGH en biopsias del periodonto de individuos sanos y con enfermedad periodontal.
Métodos: Se realizó un estudio clínico descriptivo de corte transversal en personas con diagnóstico de salud periodontal (S), gingivitis (G) y periodontitis crónica (PC). Se tomaron biopsias escisionales y se hicieron tinciones inmunohistoquímicas (hematoxilina-eosina, caspasa-3 y vimentina). Las placas se interpretaron por histopatología y se digitalizaron para cuantificar las células apoptóticas. Todos los datos se analizaron con un software estadístico para encontrar diferencias significativas (p<0.05).
Resultados: La población celular total de fibroblastos tuvo un promedio de 430±67.6 en los individuos sanos y una disminución significativamente progresiva en gingivitis (270±37.1) y periodontitis crónica (206.5±69.8) (p<0.05). La expresión de fibroblastos apoptóticos por campo aumentó de acuerdo con las severidades de la enfermedad [28±16 en sanos (6.5%); 31±17 en gingivitis (11.5%) y 51±24 en periodontitis (24.8%.), (p<0.001)]. La relación entre la expresión de fibroblastos apoptóticos y la profundidad de la bolsa periodontal no fue significante (p>0.5, r2=0.02); mientras que para las células inflamatorias se encontró una relación proporcional significativa (p<0.05, r2=0.2018).
Conclusiones: Los resultados permiten concluir que tanto los fibroblastos gingivales como las células inflamatorias presentan apoptosis manifiesta por la expresión de caspasa-3, y ésta se incrementa significativamente en gingivitis y enfermedad periodontal.

Palabras-clave
Periodontitis; Apoptosis; Gingivitis; Patogénesis; Inmunohistoquímica.

 
© Copyright 2007 - Revista Colombia Médica
Alternative site location: http://colombiamedica.univalle.edu.co

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2024, Site last up-dated on 01-Sep-2022.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Google Cloud Platform, GCP, Brazil