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Revista Colombia Médica
Universidad del Valle - Facultad de Salud
ISSN: 0120-8322
EISSN: 1657-9534
Vol. 39, No. 2, 2008, pp. 147-153
Bioline Code: rc08027
Full paper language: Spanish
Document type: Research Article
Document available free of charge

Revista Colombia Médica, Vol. 39, No. 2, 2008, pp. 147-153

 es Concordancia de métodos para susceptibilidad antimicrobiana en cepas de Mycobacterium tuberculosis check for this species in other resources aisladas en Montería, Córdoba: tubo indicador de crecimiento micobacteriano vs. método de las proporciones múltiples
Miranda, Jorge; Rios, Rodrigo; Mattar, Salim & Alvis, Nelson

Resumen

Objetivo: Evaluar la concordancia del método manual MGIT AST SIRE (mycobacteria growth indicator tube for antimicrobial susceptibility testing to isoniazid, rifampin, ethambutol and streptomycin) frente al método de proporciones múltiples (PM) para determinar susceptibilidad antimicrobiana en cepas de Mycobacterium tuberculosis.
Métodos: Se analizaron 45 cepas de M. tuberculosis aisladas en la ciudad de Montería, entre 2003 y 2005, para susceptibilidad a (INH), rifampicina (RMP), etambutol (EMB) y estreptomicina (SM). El análisis de concordancia del método MGIT manual vs PM, se realizó con la prueba Kappa.
Resultados: De las 45 cepas analizadas, 38 tuvieron idéntico resultado con ambos métodos y 7 tuvieron resultados discordantes. Los porcentajes globales de resistencia a drogas antituberculosas de las cepas analizadas por el MGIT manual y las PM fueron de 33.3% y 31.1%, respectivamente, la MDR fue 5 (11.1%) por PM y de 4 (8.8%) por MGIT manual. La concordancia del MGIT AST SIRE con el PM fue 95.5% para cada una de las drogas analizadas y kappa global de 0.8374. En cada antibiótico se encontraron dos cepas discordantes para INH, RIF (un falso resistente y un falso susceptible). En STR y EMB (dos falsos sensibles). El tiempo promedio de resultado para cualquiera de los antimicrobianos fue 6.5 días (rango 5-8 días) por MGIT, mientras que para PM fue 20 a 25 días.
Conclusión: El presente estudio mostró que MGIT manual es concordante con PM, es rápido, sencillo y eficaz, para determinar la susceptibilidad de cepas de M. tuberculosis.

Palabras-clave
M. tuberculosis; Susceptibilidad; Isoniacida; Rifampicina; Etambutol; Estreptomicina; Colombia.

 
 en Agreement between methods for antimicrobial susceptibility to Mycobacterium tuberculosis check for this species in other resources strains isolated in Montería, Córdoba: mycobacteria growth indicator tube vs. proportion method
Miranda, Jorge; Rios, Rodrigo; Mattar, Salim & Alvis, Nelson

Abstract

Objective: To evaluate the agreement of manual MGIT (mycobacteria growth indicator tube for antimicrobial susceptibility testing to isoniazid, rifampin, ethambutol and streptomycin) for susceptibility testing vs proportion method on Lowenstein-Jensen (PM) in Mycobacterium tuberculosis strains.
Methods: A total of forty-five isolates of M. tuberculosis were tested for susceptibility to isoniazid (INH), rifampin (RMP), ethambutol (EMB), and streptomycin (SM). The strains were isolated in Montería, during 2003 and 2005. The agreement between the two assays mentioned was estimated by the Kappa test.
Results: There were thirty-eight strains with identical results while 7 had discrepant results with both methods. The overall resistance to antituberculosis drugs were 33.3% y 31.1% for manual MGIT and PM, respectively. MDR was 5 (11.1%) by PM and 4 (8.8%) by manual MGIT. The agreement between MGIT AST and PM was 95.5% for all drug tested and overall kappa value 0.8374. Two discrepancies were found in each drug; with INH and RIF (one false resistant and one false susceptible). STR and EMB (two false susceptibles). Turnaround times were 5 to 8 days (median, 6.5 days) for MGIT and 20 to 25 days for MP.
Conclusions: These preliminary data show a good level of agreement between manual MGIT AST SIRE and MP. Also MGIT is a rapid, easy and efficient method for the drug susceptibility testing of M. tuberculosis.

Keywords
Mycobacterium tuberculosis; Susceptibility; Isoniazid; Rifampin; Ethambutol; Streptomycin.

 
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Alternative site location: http://colombiamedica.univalle.edu.co

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