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Revista Colombia Médica
Universidad del Valle - Facultad de Salud
ISSN: 0120-8322
EISSN: 1657-9534
Vol. 43, No. 1, 2012, pp. 38-45
Bioline Code: rc12005
Full paper language: English
Document type: Research Article
Document available free of charge

Revista Colombia Médica, Vol. 43, No. 1, 2012, pp. 38-45

 en Molecular serotyping and identification of the 85M gragment in different Colombian isolates of Listeria monocytogenes check for this species in other resources strains: A descriptive study
Vanegas, María Consuelo; Medrano, Mayra Viviana; Martínez, Aida Juliana & Arévalo, Stefany Alejandra

Abstract

Introduction: Listeria monocytogenes check for this species in other resources is a pathogen acquired through the consumption of contaminated foods. Thirteen serotypes have been reported, of which 1/2a, 1/2b, and 4b are responsible for 98% of human listeriosis cases. This study examines the association between serotypes and virulent clones, offering greater information and providing tools to prevent and control diseases caused by L. monocytogenes serotype 4b.
Objective: To identify the serotypes from L. monocytogene strains isolated from different samples by performing the molecular subtyping technique; to determine the 85M fragment that codifies for epidemic clone I.
Methods: 108 strains of L. monocytogenes were used, isolated from samples of animals, body fluids, foods, and food processing plant equipment and spaces. The samples were identified by following the Bacteriological Analytical Manual protocol described by the Food and Drug Administration (FDA). The strains were identified by Polymerase Chain Reaction (PCR) using primers and a standardized protocol from a previous research project. Serotype identification was performed by multiplex PCR. The determination of the 85M fragment of the SSCS cassette was done by following the protocol by Yildrim et al.
Results: Of the 108 L. monocytogenes strains analyzed, 60.2% (65 strains) belonged to the 4b-4d-4e serotype, 17.6% (19 strains) were identified as 1/2a-3a serotype, 14.8% (16 strains) were 4a-4c serotype, 3.7% (4 strains) belonged to the 1/2c-3c serotype, and (3.7%) corresponded to the 1/2b-3b-7 serotype. It was determined that the L. monocytogenes strains serotype 4b-4d-4e and 1/2a-3b have the 85M fragment of the SSCS cassette.
Conclusion: This study reports the predominant existence of L. monocytogenes strains serotype 4b-4d-4e in food, environmental, and clinical samples. The presence of an epidemic clone I region was also found in L. monocytogenes strains.

Keywords
Listeria monocytogenes; Listeriosis; Serotypes; 85M fragment; Isolation; Food.

 
 es Serotipificación molecular e identificación del fragmento 85M de aislamientos colombianos de Listeria monocytogenes check for this species in other resources : Un estudio descriptivo
Vanegas, María Consuelo; Medrano, Mayra Viviana; Martínez, Aida Juliana & Arévalo, Stefany Alejandra

Resumen

Introducción: Listeria monocytogenes check for this species in other resources es un patógeno adquirido por el consumo de alimentos contaminados. Se han reportado 13 serotipos de los cuales 1/2a, 1/2b y 4b son responsables del 98% de las listeriosis humanas. Se ha estudiado la asociación entre serotipos y clones de virulencia, lo cual aporta información y brinda herramientas para la prevención y control de las enfermedades causadas por L. monocytogenes serotipo 4b.
Objetivo: Identificar los serotipos de L. monocytogenes aisladas de diferentes fuentes y determinar la presencia del fragmento 85M que codifica para clon epidémico I.
Métodos: Aislamiento de L. monocytogenes a partir de muestras animales, fluidos corporales, comestibles, equipos y ambientes en plantas de alimentos, según el protocolo FDA. Identificación por reacción en cadena de la polimerasa (PCR), serotipificación molecular por PCR multiplex y determinación del fragmento 85M según Yildrim et al.
Resultados: De las 108 cepas de L. monocytogenes serotipificadas molecularmente, 60.2% (65 cepas) pertenecen al serotipo 4b-4d-4e, el 17.6% (19 cepas) fueron 1/ 2a-3a, el 14.81% (16 cepas) son serotipo 4a-4c, el 3.7% (4 cepas) corresponden a 1/2c-3c y el 3.7% fueron aislamientos serotipo 1/2b-3b-7. Se determinó que todas las cepas serotipo 4b-4d-4e y 1/2a-13b contienen el fragmento 85M del clon epidémico I.
Conclusión: Este estudio reporta la existencia predominante de cepas de L. monocytogenes serotipo 4b-4d-4e en muestras de alimentos, ambientes y otras. Asimismo, evidencia la presencia de una región del fragmento 85M del clon epidémico I de L. monocytogenes en aislamientos pertenecientes al serotipo con más frecuencia relacionado con brotes de listeriosis en humanos (4b).

Palabras-clave
Listeria monocytogenes; Listeriosis; Serotipos; Fragmento 85M; Serotipos; Aislamiento; Alimentos.

 
© Copyright 2012 Colombia Médica
Alternative site location: http://colombiamedica.univalle.edu.co

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