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Revista Colombia Médica
Universidad del Valle - Facultad de Salud
ISSN: 0120-8322
EISSN: 1657-9534
Vol. 43, No. 3, 2012, pp. 184-188
Bioline Code: rc12030
Full paper language: English
Document type: Research Article
Document available free of charge

Revista Colombia Médica, Vol. 43, No. 3, 2012, pp. 184-188

 en Identification of messenger RNA of fetoplacental source in maternal plasma of women with normal pregnancies and pregnancies with intrauterine growth restriction
Paola, Ayala Ramírez; García Robles, Reggie; Rojas, Juan Diego; Bermúdez, Martha & Bernal, Jaime

Abstract

Objective: to quantify placenta-specific RNA in plasma of women carrying foetuses with intrauterine growth restriction and pregnant women with normal pregnancies.
Materials and methods: 8 pregnant women with foetuses with intrauterine growth restriction were studied as well as 18 women with uncomplicated pregnancies in the third pregnancy trimester. Total free RNA was quantified in maternal plasma by spectrophotometry and the gene expression of hPL (Human Placental Lactogen) at the messenger RNA level through technical Real Time-Chain Reaction Polymerase.
Results: plasma RNA of fetoplacental origin was successfully detected in 100% of pregnant women. There were no statisti- cally significant differences between the values of total RNA extracted from plasma (p = 0.5975) nor in the messenger RNA expression of hPL gene (p = 0.5785) between cases and controls.
Conclusion: messenger RNA of fetoplacental origin can be detected in maternal plasma during pregnancy.

Keywords
RCIU, Messenger RNA, plasma, pregnancy complications, human placental lactogen, Chain Reaction Reverse Transcriptase Polymerase

 
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