The status of msp1
and the diversity of Plasmodium falciparum
Colombia before the implementation of an artemisinin-combined
therapy have been explored only by a few authors and in a relatively
small number of samples from this highly endemic region.
To evaluate the frequency of msp1
, and glurp
alleles and the diversity of P. falciparum
in two Colombian regions
before the use of an artemisinin-combined therapy.
This study was part of a major anti-malarial efficacy trial
designed as a random, clinically-controlled study for which 224
subjects were recruited. Region 2 of msp1
were amplified by a nested PCR; glurp
(region R2) was amplified
by a semi-nested PCR.
, five genotypes were observed, representing the
K1, MAD20, and RO33 allelic families. All samples corresponded
to a MAD20 150 bp allele. For msp2
(IC family), two alleles were
detected and for glurp
, eight were observed. A total 33 haplotypes
Analysis of glurp
can be used to successfully genotype
parasite populations in the new studies in Colombia aimed
at exploring Plasmodium spp.
population dynamics. In addition,
analysis of msp1
can also be of value for comparisons
with past studies, but not when the objective is to study parasites
obtained from the same patient in a reduced period of time; for
instance, during treatment efficacy studies.