The effect of culture medium volume on in vitro development of mouse embryos|
Malekshah, Abbasali Karimpor & Moghaddam, Amir Esmailnejad
In the field of mammalian embryo culture, the putative influence of autocrine/ paracrine factor(s), produce by the embryos itself, is under investigation. A smaller medium drop can prevent dilution of this factor(s).
The objective of this study was to examine the effect of culture medium volume on in vitro development of mouse 2-cell embryos.
Materials and Methods:
The embryos were obtained from female NMRI mice. To evaluate the effect of medium volume, groups of 16-20 late 2-cell embryos were cultured in 2, 5, 10, 20, 50 and 100 µl of drops of Ham's F10 medium for 72 h.
Development to blastocyst stage in 50 and 100 µl of drop were significantly higher than this in any other volume (p<0.001). Almost a similar pattern was also observed for hatched blastocyst formation. However, the total number of cells in blastocysts, developing in different volumes, were not significantly different.
These results indicate that the optimal volumes of Ham's F10 medium for mouse early embryo development are 50 to 100 µl. However, volumes as small as 2 µl can successfully support mouse 2-cell embryo development to blastocyst and hatching stages.
Mouse embryo, Culture medium, Incubation volume