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Iranian Journal of Reproductive Medicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
ISSN: 1680-6433
EISSN: 2008-2177
Vol. 5, No. 4, 2007, pp. 165-170
Bioline Code: rm07033
Full paper language: English
Document type: Research Article
Document available free of charge

Iranian Journal of Reproductive Medicine, Vol. 5, No. 4, 2007, pp. 165-170

 en Evaluation of epididymal necrospermia following experimental chronic spinal cord injury in rat
Talebi, Ali Reza; Khalili, Mohammad Ali; Nahangi, Hossein; Abbasi, Abulghasem & Anvari, Morteza

Abstract

Background: Spinal cord injury (SCI) occurs most often to young men at the peak of their reproductive health. Only 10% of SCI men can father children without medical assistance due to potential impairments in ejaculation and sperm quality.
Objective: The main objective of this experimental study was to evaluate the epididymal necrospermia- sperm death, after chronic SCI in rat.
Materials and Methods: Forty-five adult Wistar rats were divided into 3 groups of SCI, sham, and control. Following laminectomy, SCI was induced onto exposed dura matter (T10) of anesthetized rats. Sham group underwent laminectomy of T10 only; while, control rats were not exposed to any type of injury or medication. The spermatozoa from cauda epididymis were aspirated after 50 days for analysis of necrospermia with three assays of Eosin-Y staining, Hypo-osmotic swelling (HOS), and Hoechst 33258 fluorescent dye.
Results: The rate of necrospermia in SCI rats was significantly increased when compared with other groups (p<0.05). Also, the rates of necrspermia in SCI samples were similar with application of 3 assays (Eosin-Y: 46.11±9.41; HOS: 45.88±8.89; Hoechst: 46.76±9.31). Total necrospermia was not observed in any of the epididymal samples.
Conclusion: The results showed that chronic SCI is associated with high rate of epididymal necrospermia in mammals such as rats. It is, therefore, recommended that an effective laboratory technique, such as Hoechst 33258 should be used for separation of live and motile sperms from necrospermic ones for assisted reproduction program.

Keywords
Spinal cord injury, Spermatozoa, Necrospermia, Rat.

 
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