Iranian Journal of Reproductive Medicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
Vol. 6, No. 1, 2008, pp. 13-18
Bioline Code: rm08003
Full paper language: English
Document type: Research Article
Document available free of charge
Iranian Journal of Reproductive Medicine, Vol. 6, No. 1, 2008, pp. 13-18
© Copyright 2008 - Iranian Journal of Reproductive Medicine
Correlation between sperm parameters and sperm DNA fragmentation in fertile and infertile men|
Sheikh, Nasrin; Amiri, Iraj; Farimani, Marzieh; Najafi, Rezvan & Hadeie, Jafar
It is established that sperm DNA integrity is essential in fertilization and
normal embryo and fetal development. Routine semen analysis gives an approximate
evaluation of the functional competence of spermatozoa, but does not always reflect the
quality of sperm DNA. Therefore, the evaluation of sperm DNA integrity, in addition to
routine sperm parameters, could add further information on the quality of spermatozoa
and reproductive potential of males.
The objective of this study was to determine the levels of sperm DNA
damage in fertile and infertile males and its correlation with semen parameters.
Materials and Methods:
Semen samples were collected from 45 infertile men selected
from couples attending the infertility clinic with a history of infertility of ≥1 years and
75 healthy volunteers of proven fertility (initiated a successful pregnancy) served as the
control group. After routine sperm analysis, DNA damage was determined using single
cell gel electrophoresis (comet) assay method.
The mean of DNA damage (comet value) in the sperms of infertile males was
significantly higher than that of fertile males (12.9±7.59 vs. 48.77±24.42, p<0.001). A
significant negative correlation was observed between DNA damage and sperm motility
in fertile group (p<0.02, R=-0.263). In infertile males, significant negative correlations
were observed between DNA damage with sperm motility (p<0.002, R=-0.45) and
morphology (p<0.03, R=-0.317). There was no significant correlation between sperm
concentration and sperm DNA damage in both groups.
These results indicate that sperm DNA damages in infertile males is
significantly higher than fertile males and sperms with abnormal morphology and low
levels of motility has more abnormal DNA damages than motile and normal sperms.
Sperm parameters, Male infertility, DNA integrity, Comet assay.
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