International Journal of Reproductive Medicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
Vol. 6, No. 2, 2008, pp. 65-69
Bioline Code: rm08011
Full paper language: English
Document type: Research Article
Document available free of charge
International Journal of Reproductive Medicine, Vol. 6, No. 2, 2008, pp. 65-69
© Copyright 2008 - Iranian Journal of Reproductive Medicine
Reactive oxygen species (ROS) production in seminal fluid correlate with the severity of varicocele in infertile men|
Moein, Mohammad Reza; Soleimani, Mehrdad & Tabibnejad, Nasim
Reactive oxygen species (ROS) are a group of free radicals that in
excessive amounts have negative influence on sperm quality and function.
To study the effect of varicocele and its severity on the level of ROS in
infertile men with clinical varicocele.
Materials and Methods:
In this controlled prospective study, 42 men with clinically
diagnosed left varicocele and 30 fertile men were studied. Patients were asked about
history of urogenital infection, using any antioxidant medication and smoking. Grade of
varicocele was determined by physical examination. Levels of ROS in seminal plasma
were measured in each group by a chemiluminiscence assay. The sperm parameters
were also determined and compared in different groups.
The ROS levels were significantly higher in patients with varicocele than
normal men (mean: 1575.42 RLU (Radio Luminescence Unit) vs. 53.79 RLU,
p=0.005). In total 20 patients had grade I, 20 patients grade II and 2 patients had grade
III varicocele. The mean ROS levels were 669.12 RLU, 2406.87 RLU and 2324 RLU in
patients with grade I, II and III varicocele respectively (p=0.144). In case group, 15
patients were smoker and 27 were non-smokers. The mean ROS levels in patients with
the history of smoking was 1367.71 RLU while in non-smokers it was 897.672 RLU
Our study showed that increased levels of ROS production in the seminal
fluid can be an important factor in the etiology of male infertility in patients with
varicocele, and this effect is more prominent with higher grade of varicocele.
Male infertility, Varicocele, Reactive Oxygen Species (ROS), Smoking.
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