International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
Vol. 6, No. 3, 2008, pp. 125-131
Bioline Code: rm08021
Full paper language: English
Document type: Research Article
Document available free of charge
International Journal of Reproductive BioMedicine, Vol. 6, No. 3, 2008, pp. 125-131
© Copyright 2008 - Iranian Journal of Reproductive Medicine
The changes in morphology and morphometrical indices of endometrium of ovariectomized mice in response to exogenous ovarian hormones|
Peyghambari, Fatemeh; Salehnia, Mojdeh; Moghadam, Mehdi Forouzandeh; Valujerdi, Mojtaba Rezazadeh & Hajizadeh, Ebrahim
The preparation of endometrium for embryo reception and implantation
are controlled by ovarian hormones. These hormones have distinct cyclical changes
during estrus cycle.
The aim of this study was to evaluate the changes in morphology and
morphometrical indices of endometrium by daily injections of estrogen and
progesterone in ovariectomized mouse.
Materials and Methods:
In total 60 adult NMRI female mice were ovariectomized and
after two weeks, they were randomly divided into five groups: control, sham group,
estrogen treated mice (which received daily dosage of 0.5 ml/mouse of hormone for five
days), progesterone treated mice (which received daily dosage of 0.2 ml/mouse of
progesterone hormone for five days) and estrogen-progesterone treated mice (they
received 0.5 ml/mouse estrogen on the first day and 0.2 ml/mouse progesterone
injections from the second day to the fifth day of treatment). The mice were sacrificed
in every day (n=5) up to five days after treatment and their uterine horns were obtained
and processed for morphological and morphometrical studies.
On the second day of treatment, the diameter of glands was observed to be
more in the progesterone group (53.75±6.32μ) than this in the estrogen (45.13±7.78 μ)
and estrogen-progesterone treated groups (48.17±13.58 μ). While, the number of glands
(76.25±17.37) and thickness of endometrium (39.58±3.37 μ) were observed to be more
in the estrogen treated group (p=0.01).
Progesterone had effect on the gland whereas estrogen caused increased in
height of surface epithelium of endometrium. Overall, the day 2 after treatment (in all
experimental groups) is suitable day for sampling for further studies.
Endometrium, Exogenous ovarian hormones, Ovariectomized mice, Morphometry, Morphology.
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