International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
Vol. 6, No. 3, 2008, pp. 133-142
Bioline Code: rm08022
Full paper language: English
Document type: Research Article
Document available free of charge
International Journal of Reproductive BioMedicine, Vol. 6, No. 3, 2008, pp. 133-142
© Copyright 2008 - Iranian Journal of Reproductive Medicine
Morphological and morphometrical changes of endometrim after application of estrogen and progesterone during luteal phase in the superovulated mice|
Niknafs, Behrooz; Afshari, Fathemeh & Dezfulian, Abdo-rahman
The application of luteal phase supplementation hormones to increase the
implantation rate is on debate among researchers.
In this study, the morphological and morphometrical assessment of
superovulated mice endometrium were investigated at window implantation period
Materials and Methods:
Female mice were superovualated then were mated with
vasectomized mice; the mice were divided in experimental and control groups.
Experimental group included five groups which in them pseudopregnant mice were
given a four consecutive daily injection of 1-progesterone (P), 2-estrogen (E), 3-
estrogen + progesterone, 4-antiprogesterone+estrogen and 5-sham group. The uterine of
all groups were collected after 4.5 day of pseudopregnancy and were prepared for
histological and morphometrical studies.
Morphological studies of endometrial tissue showed that the luminal
epithelium in group P appeared cuboidal shape. Endometrial folding was very high in
group E+P. The luminal epithelium in groups E, E+P and RU 486 + E were seen in
different morphology in comparison to control group. Morphometrical evaluation also
showed height of luminal epithelium in group E (32.7±0.67) and E+P (33.6±1.3) were
higher than those were seen in control (22.5±1.5) and group P (15.3±1.2).
Progesterone caused the lowest endometrial development compare to other
groups. It is concluded that the adding of E to P may improve endometrial condition to
implant at luteal phase.
Mice, Morphometry, Estrogen, Progesterone, Luteal phase.
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