|
International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
ISSN: 1680-6433 EISSN: 1680-6433
Vol. 7, No. 2, 2009, pp. 53-58
|
Bioline Code: rm09010
Full paper language: English
Document type: Research Article
Document available free of charge
|
|
International Journal of Reproductive BioMedicine, Vol. 7, No. 2, 2009, pp. 53-58
en |
Relationships between free leptin and insulin resistance in women with polycystic ovary syndrome
Mohiti-Ardekani, Javad; Tarof, Nasim & Aflatonian, Abbas
Abstract
Background:
Leptin is an adipokine that circulates in a free form and bound to a
soluble leptin receptor. Patients with polycystic ovary syndrome have increased insulin
resistance and high incidence of obesity.
Objective:
This study was carried out to evaluate levels of leptin and free leptin in
women with polycystic ovarian syndrome (PCOS) and note any relationships with
insulin resistance and adiposity.
Materials and Methods:
We assessed the correlation of metabolic parameters with the
levels of free leptin and it’s bound form in 27 PCOS women (aged 26±5.6 years) and 27
healthy women with normal menstrual cycle as controls (aged 25 ±4 years).Total leptin
and insulin levels were measured using ELISA. Free leptin form was purified by Gel
filtration chromatography and their collected fractions were measured by a sensitive
ELISA-Kit. Insulin resistance was calculated by homeostasis model assessment
(HOMA).
Results:
In PCOS patients and control group a correlation between leptin and body
mass index (BMI) was found. A significant difference was found between leptin and
free leptin levels in PCOS subjects and controls (p<0.05). Significant correlations were
found between free and total leptin with insulin resistant in PCOS subjects (r=0.78
p=0.00, r=0.84 p=0.003) and control groups respectively (r=0.86 p=0.00, r=0.69
p=0.00).
Conclusion:
Total and free leptin forms are correlated significantly with BMI in
patients with PCOS and in controls. Total and free leptin forms showed significant
correlations with insulin resistance but no significant difference was seen in the two
groups investigated.
Keywords
Free leptin, Polycystic Ovarian Syndrome (PCOS), Insulin resistance
|
|
© Copyright 2009 - Iranian Journal of Reproductive Medicine Alternative site location: http://www.ijrm.ir
|
|