|
International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
ISSN: 1680-6433 EISSN: 1680-6433
Vol. 7, No. 2, 2009, pp. 73-77
|
Bioline Code: rm09013
Full paper language: English
Document type: Research Article
Document available free of charge
|
|
International Journal of Reproductive BioMedicine, Vol. 7, No. 2, 2009, pp. 73-77
en |
Can Zeta sperm selection method, recover sperm with higher DNA integrity compare to density gradient centrifugation?
Khajavi, Noush Afarin; Razavi, Shahnaz; Mardani, Mohammad; Tavalaee, Marziyeh; Deemeh, Mohammad Reza & Nasr-Esfahani, Mohammad Hossein
Abstract
Background:
Sperm selection for ICSI based on morphology and motility might not be
relevant to chromatin integrity. Thus sperm selection based on sperm characteristics has
been suggested.
Objective:
The aim of this study was to compare the efficiency of Zeta method with
routine Density Gradient Centrifugation method (DGC) for the selection of sperm with
higher DNA integrity.
Materials and Methods:
Semen samples were obtained from 63 individuals referring
to Andrology Unit of Isfahan Fertility and Infertility Center. Semen analysis was carried
out according to WHO criteria. Each semen sample was divided into three equal
portions. One portion was used as control, the second portion was used for Zeta method
and the third portion underwent DGC method. Each portion was evaluated to DNA
integrity by TUNEL assay. Student t-test was carried out using SPSS and p-value lower
than 0.05 was considered significant.
Results:
The mean number of sperm DNA fragmentation in Zeta and DGC methods
were significantly decreased compare to the control group (p<0.001). In addition, Zeta
method was more efficient than the DGC method in the selection of sperm with intact
DNA (p<0.001).
Conclusion:
The Zeta method appears to be a suitable procedure to recover sperm with
normal DNA integrity.
Keywords
Zeta method, Density Gradient Centrifugation method, DNA fragmentation
|
|
© Copyright 2009 - Iranian Journal of Reproductive Medicine Alternative site location: http://www.ijrm.ir
|
|