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Iranian Journal of Reproductive Medicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
ISSN: 1680-6433
EISSN: 2008-2177
Vol. 10, No. 3, 2012, pp. 181-192
Bioline Code: rm12027
Full paper language: English
Document type: Research Article
Document available free of charge

Iranian Journal of Reproductive Medicine, Vol. 10, No. 3, 2012, pp. 181-192

 en Histological and histochemical effects of Gly-phosate on testicular tissue and function
Razi, Mazdak; Najafi, Golamreza; Feyzi, Sajad; Karimi, Ali; Shahmohamadloo, Simineh & Nejati, Vahid

Abstract

Background: In this study we aimed to evaluate the impact of chronic exposure to the Gly-phosate (GP) on rat’s testicular tissue and sperm parameters.
Objective: Testicular tissue, morphology of sperms and testosterone level in serum of mature male rats were analyzed.
Materials and Methods: Animals were divided into two test and control-sham groups. The test group was subdivided into 4 groups (10, 20, 30 and 40 days GP administrated). Each test group (n=8) received the compound at dose of 125 mg/kg, once a day, orally for 40 days while control-sham group (n=16) received the corn oil (0.2 ml/day).
Results: Microscopic analyses revealed increased thickness of tunica albuginea, obvious edema in sub-capsular and interstitial connective tissue, atrophied seminiferous tubules, arrested spermatogenesis, negative tubular differentiation and repopulation indexes, decreased Leydig cells/mm2 of interstitial tissue, hypertrophy and cytoplasmic granulation of Leydig cells, elevated death, immature sperm and increased immotile and abnormal sperm percentage. The carbohydrate ratio was reduced in first three layers of the germinal epithelium (GE) cytoplasm. The upper layers of the GE series were manifested with low rate of lipid accumulation in cytoplasm, while the cells which were located in first layers were revealed with higher amount of lipid foci. Hematological investigations showed significant (p <0.05) decreasing of testosterone level in serum.
Conclusion: The current data provide inclusive histological feature of chronic exposure against GP with emphasizing on reproductive disorders including histological adverse effect on the testicular tissue, spermatogenesis, sperm viability and abnormality which potentially can cause infertility.

Keywords
Abnormal sperm, Carbohydrate accumulation, Gly-phosate, Lipid foci, Spermatogenesis, Testosterone, Testis.

 
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Alternative site location: http://www.ijrm.ir

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