Iranian Journal of Reproductive Medicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
Vol. 10, No. 6, 2012, pp. 555-560
Bioline Code: rm12080
Full paper language: English
Document type: Research Article
Document available free of charge
Iranian Journal of Reproductive Medicine, Vol. 10, No. 6, 2012, pp. 555-560
© Copyright 2012 - Iranian Journal of Reproductive Medicine
Effects of prenatal exposure to extremely low electro-magnetic field on in vivo derived blastocysts of mice|
Bayat,Parvin-Dokht; Darabi, Mohmmad; Ghanbari , Ali; Amiri , Sara & Sohouli, Pardis
Background: Indisputable population exposure to widespread electromagnetic fields, has grown concerns over the probable health effects of these fields.
Objective: The present study was aimed to examine the possible effects of 50 Hz extremely low frequency electromagnetic field (ELF-EMF) exposure on the number and quality of mice blastocysts.
Materials and Methods: In present study, 66 NMRI pregnant females divided into two treated and non-treated groups. The treated group exposed to ELF-EMF (50 Hz and 6×10-3 T). Subsequently, embryos were collected by flushing the uterine horn and Fallopian tubes on the day 3 of gestation. Number of trophoectoderm (TE) and Inner Cell Mass (ICM) cells in blastocysts were determined after differential nuclei staining using a modified method. Furthermore, number of all flushed blastocysts calculated in each group.
Results: There was no significant difference in mean number of blastocysts in treated (6.64±1.34) and none treated (8.22±1.59) groups. In treated group, there were significant decreased in total cell number of blastocysts (p=0.000), number of ICM cells (p=0.000), and number of TE cells (p=0.001) whereas the ratio of ICM/TE cells increased (p=0.002).
Conclusion: The data indicate that ELF-EMF is able to affect cellular composition of blastocysts, but it can't omit total volume of blastocysts.
Blastocyst, Electromagnetic fields, Mice
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