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Iranian Journal of Reproductive Medicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
ISSN: 1680-6433
EISSN: 2008-2177
Vol. 11, No. 9, 2013, pp. 767-771
Bioline Code: rm13097
Full paper language: English
Document type: Short Communication
Document available free of charge

Iranian Journal of Reproductive Medicine, Vol. 11, No. 9, 2013, pp. 767-771

 en Effect of zinc oxide nanoparticles on viability of human spermatozoa
Barkhordari, Abolfazl; Hekmatimoghaddam, Seyedhossein; Jebali, Ali; Khalili, Mohammad Ali; Talebi, Alireza & Noorani, Marzieh

Abstract


Background: The extensive use of different nanoparticles has raised great concerns about their occupational and biological safety.
Objective: The aim of this study was to evaluate the cytotoxic effect of zinc oxide nanoparticles (ZnO NPs) on viability of spermatozoa.
Materials and Methods: Semen samples were obtained from 15 healthy persons, and were analyzed using WHO guidelines. Each semen sample was separately incubated with different concentrations of ZnO NPs (10, 100, 500, and 1000 μg/mL) at 37PoPC for 45, 90, and 180 minutes. Then, the cell death percentage of spermatozoa was measured by MTT assay. Mann-Whitney test was used for comparison of different times and concentrations.
Results: The maximum cell death percentage was 20.8%, 21.2%, and 33.2% after 45, 90, and 180 minutes, respectively. In case of concentration, the highest concentration (1000 μg/mL) of ZnO NPs led to the highest toxicity for all incubation times. Statistically, there were significant differences in cell viability after 180 minutes vs. 45 and 90 minutes.
Conclusion: This study indicated that cytotoxicity of ZnO NPs is dose and time dependent.

Keywords
Spermatozoa; Viability; MTT assay; ZnO nanoparticles; Semen; Cytotoxicity

 
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Alternative site location: http://www.ijrm.ir

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