International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
Vol. 12, No. 3, 2014, pp. 209-216
Bioline Code: rm14028
Full paper language: English
Document type: Research Article
Document available free of charge
International Journal of Reproductive BioMedicine, Vol. 12, No. 3, 2014, pp. 209-216
© Copyright 2014 - Iranian Journal of Reproductive Medicine
Protective effect of royal jelly on fertility and biochemical parameters in bleomycin-induced male rats|
Amirshahi, Tayebeh; Najafi, Gholamreza & Nejati, Vahid
Background: Bleomycin (BL) is a glycopeptide antibiotic obtained from the bacterium Streptomyces verticillus which is routinely used for treatment of human cancers. Royal jelly (RJ) is a production from the hypo pharyngeal, mandibular and post cerebral glands of nurse bees. RJ consists of 66% water, 15% sugars, 5% lipids, and 13% proteins, essential amino acids and vitamins.
Objective: The aim of present study was to evaluate protective effect of royal jelly on sperm parameters and malondialdehyde (MDA) production in rat.
Materials and Methods: Forty adult male wistar rats (220±20gr) were randomly
divided into 4 groups (n=10). Control group (CG) received normal saline 10 ml/kg
twice a week with Intraperitoneal (I.P) for 48 days (0.3 ml/rat). Royal Jelly group
(RJG) received jelly (100 mg/kg daily) for 48 days orally. Bleomycin group (BLG)
received BL (10 mg/kg twice a week) with I.P for 48 days. Royal Jelly+ Bleomycin
group (RJ+BLG) received royal Jelly (100 mg/kg /day) orally concomitant with BL
administration. Sperm count, motility, and viability were investigated and chromatin
quality and DNA integrity were also analyzed. Serum testosterone and MDA
concentrations were measured as well.
Results: BL caused decline significantly (p<0.05) sperm count, sperm viability, motility as well as testosterone concentration compared to control group while significant (p<0.05) increases in immature sperm, sperm with damaged DNA and MDA concentration were announced in BL in comparison with CG and RJ+BLG. Royal jelly improved Bleomycin-induced toxicity on sperm parameters and testosterone and MDA concentrations.
Conclusion: The present results support the idea that BL adversely affects sperm parameters and MDA and the RJ with antioxidant properties has positive effects on these parameters.
Bleomycin; Royal jelly; Testis; Testosterone; Rat
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