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Iranian Journal of Reproductive Medicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
ISSN: 1680-6433
EISSN: 2008-2177
Vol. 12, No. 10, 2014, pp. 681-686
Bioline Code: rm14095
Full paper language: English
Document type: Research Article
Document available free of charge

Iranian Journal of Reproductive Medicine, Vol. 12, No. 10, 2014, pp. 681-686

 en Altered of microRNA expression level in oligospermic patients
Abhari, Alireza; Zarghami, Nosratollah; Farzadi, Laya; Nouri, Mohammad & Shahnazi, Vahideh

Abstract


Background: MicroRNA (miRNA) is small endogenous, single strand RNA molecules that regulate gene expression at post-transcriptional level through several mechanisms to affect key cellular event including male germ cells differentiation, proliferation, development and apoptosis. Mutation and/or aberrant expression of miRNAs have been associated with progression of various disorders, including infertility.
Objective: The purpose of this research was to study the estrogen receptor beta (ERβ), hsa-mir-21 and, hsa-mir-22 expression level in oligospermic infertile and control fertile men and correlation between them.
Materials and Methods: In this study, the change in mir-21, mir-22 expression and their common target gene (ERβ) expression levels were evaluated in oligospermic infertile men (n= 43) compared with 43 age matched healthy control by Real-Time PCR methods.
Results: Expression analysis by qRT-PCR test on miRNA have identified that mir-21, mir-22 levels were significantly higher than those in normal controls (p<0.0001) and ERβ expression level significantly decreased in comparison with the normal group (p<0.0001).
Conclusion: Our study showed that mir-21 and mir-22 are indirectly involved in spermatogenesis by regulating of the estrogen receptor and might have a diagnostic and prognostic value in men infertility.

Keywords
mir-21; mir-22; microRNA; Fertility; Oligospermia

 
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Alternative site location: http://www.ijrm.ir

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