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Iranian Journal of Reproductive Medicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
ISSN: 1680-6433
EISSN: 2008-2177
Vol. 13, No. 5, 2015, pp. 269-274
Bioline Code: rm15038
Full paper language: English
Document type: Research Article
Document available free of charge

Iranian Journal of Reproductive Medicine, Vol. 13, No. 5, 2015, pp. 269-274

 en DNA flow cytometric analysis in variable types of hydropic placentas
Pasdar, Fatemeh Atabaki; Khooei, Alireza; Fazel, Alireza; Rastin, Maryam; Tabasi, Nafise; Peirouvi, Tahmineh & Mahmoudi, Mahmoud

Abstract

Background: Differential diagnosis between complete hydatidiform mole, partial hydatidiform mole and hydropic abortion, known as hydropic placentas is still a challenge for pathologists but it is very important for patient management.
Objective: We analyzed the nuclear DNA content of various types of hydropic placentas by flowcytometry.
Materials and Methods: DNA ploidy analysis was performed in 20 non-molar (hydropic and non-hydropic spontaneous abortions) and 20 molar (complete and partial moles), formalin-fixed, paraffin-embedded tissue samples by flow cytometry. The criteria for selection were based on the histopathologic diagnosis.
Results: Of 10 cases histologically diagnosed as complete hydatiform mole, 9 cases yielded diploid histograms, and 1 case was tetraploid. Of 10 partial hydatidiform moles, 8 were triploid and 2 were diploid. All of 20 cases diagnosed as spontaneous abortions (hydropic and non-hydropic) yielded diploid histograms.
Conclusion: These findings signify the importance of the combined use of conventional histology and ploidy analysis in the differential diagnosis of complete hydatidiform mole, partial hydatidiform mole and hydropic abortion.

Keywords
Flow cytometry; Hydatidiform mole; Spontaneous abortion; Ploidy

 
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