Administration of high dose of methamphetamine has detrimental effects on sperm parameters and DNA integrity in mice|
Sabour, Mojdeh; Khoradmehr, Arezoo; Kalantar, Seyyed Mehdi; Danafar, Amir Hossein; Omidi, Marjan; Halvaei, Iman; Nabi, Ali; Ghasemi-Esmailabad, Saeed & Talebi, Ali Reza
Background: Methamphetamine (MA) was shown to have harmful effects on male
Objective: To investigate probable effects of daily administration of MA on sperm
parameters and chromatin/DNA integrity in mouse.
Material and Methods: Thirty-five NMRI male mice were divided into five groups
including low, medium, and high dosage groups which were injected
intraperitoneally with 4, 8 and 15 mg/kg/day for 35 days, respectively. Normal
saline was injected in sham group and no medications were used in control group.
Then, the mice were killed and caudal epididymis of each animal was cut and placed
in Ham’s F10 medium for sperm retrieval. To evaluate sperm chromatin
abnormalities, the aniline blue, toluidine blue and chromomycine A3 were used. For
sperm DNA integrity and apoptosis, the acridine orange, sperm chromatin
dispersion, and TUNEL assay were applied. For sperm morphology, Papanicolaou
staining was done
Results: Normal morphology and progressive motility of spermatozoa decreased in
medium and high dosage groups in comparison with the control group (p=0.035).
There was a significant increase in rate of aniline blue, toluidine blue, and
chromomycine A3 positive spermatozoa in high dosage group. In a similar manner,
there was an increase in rates of acridine orange, TUNEL and sperm chromatin
dispersion positive sperm cells in high dosage group with respect to others.
Conclusion: MA abuse in a dose-dependent manner could have detrimental effects
on male reproductive indices including sperm parameters and sperm
chromatin/DNA integrity in mice.
Methamphetamine; Apoptosis; Sperm; Sperm chromatin