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International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
ISSN: 1680-6433 EISSN: 1680-6433
Vol. 16, No. 8, 2018, pp. 519-528
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Bioline Code: rm18062
Full paper language: English
Document type: Research Article
Document available free of charge
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International Journal of Reproductive BioMedicine, Vol. 16, No. 8, 2018, pp. 519-528
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The effect of crocin on testicular tissue and sperm parameters of mice offspring from mothers exposed to atrazine during pregnancy and lactation periods: An experimental study
Fani, Masoumeh; Mohammadipour, Abbas & Ebrahimzadeh-Bideskan, Alireza
Abstract
Background: Atrazine as a herbicide may affect the human’s health. Crocin may
protect atrazine-induced damages.
Objective: The aim of this study was to evaluate the effects of atrazine on mice
testicular tissue and sperm parameters and protective effects of Crocin on probably
atrazine-induced damages.
Materials and Methods: in this experimental study, 24 pregnant Balb/c mice were
randomly divided to 4 groups: I: Atrazine (10 mg/kg), II: Atrazine-Crocin, III:
Crocin (10mg/kg) and IV: Normal saline. Administrations were done daily by
gavage during pregnancy and lactation. In the end, two male offspring were
randomly selected from every mother and sacrificed respectively on 23 and 75
postnatal days. Then, their epididymides were removed for sperm parameters
investigation and their testes were prepared to evaluate apoptosis by means of
TUNEL technique.
Results: The mean number of sperms in the atrazine group was lower compared to
other groups and increased in the atrazine-crocin group compared with atrazine
group significantly (p=0.001). Sperm abnormality was increased in the atrazine
group compared with the normal saline group and decreased in the atrazine-crocin
group compared with atrazine group significantly (p≤0.001). TUNEL-positive
spermatogonia in 23 days old offspring increased significantly in the atrazine group
compared with other groups (p=0.03). TUNEL-positive spermatogenic cells in 75
days old offspring was significantly increased in the atrazine group compared with
the saline group (p≤0.001).
Conclusion: Atrazine exposure may lead to decrease the number of sperms, increase
sperms abnormality, spermatogenic cell apoptosis and height of germinal
epithelium. These complications may improve by crocin administration.
Keywords
Atrazine; Crocin; Apoptosis; Spermatogenesis; Mouse.
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