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International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
ISSN: 1680-6433 EISSN: 1680-6433
Vol. 16, No. 9, 2018, pp. 601-608
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Bioline Code: rm18072
Full paper language: English
Document type: Case Report
Document available free of charge
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International Journal of Reproductive BioMedicine, Vol. 16, No. 9, 2018, pp. 601-608
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Chromosome segregation and chromatin integrity in spermatozoa from a t(2;8)(p24;p21)mat carrier: A case-report
García-Vielma, Catalina; Dávila-Rodríguez, Martha Imelda & Cortés-Gutiérrez, Elva Irene
Abstract
Background: Chromosome rearrangements can produce genomic imbalance in
gametes which causes a drastic decrease in fertility. Several studies have described
the relationship between high levels of DNA damage and chromosomal alterations
in the spermatozoa of infertile or subfertile males. However, the nature of this
relation is poorly understood. In this study, the meiotic segregation pattern and
chromatin integrity were analyzed in the ejaculated spermatozoa of a 46, XY,
t(2;8)(p24;p21)mat carrier with normozoospermia and a lack of conception.
Case: A 39-year-old infertile man with a 46, XY, t(2;8)(p24;p21)mat inherited from
his mother, was studied. The wife of the proband (30 yrs old) had a normal
karyotype and no reproductive problems. The meiotic segregation pattern and
aneuploidy of chromosome-8 and chromosome-2 were analyzed by FISH. Sperm
DNA damage was evaluated by the Sperm Dispersion Chromatin, alkaline comet
assay and DNA breaking detection. Five healthy male donors were included as
controls. The frequency of genetically unbalanced spermatozoa was 61.6%. Analysis
of the aneuploidy of chromosome-8 and chromosome-Y revealed approximately
three and 24 fold increased level respectively in comparison with that of the control
group.
Conclusion: We suggest that the accumulation of genetically unbalanced
spermatozoa, and increased sperm aneuploidy level is related to male infertility.
Interestingly, the case described here has a high level of sperm chromosomal
imbalance appears to be linked to sperm DNA fragmentation status. This
information could be useful in assisted reproductive techniques.
Keywords
Infertility; Male; Spermatozoa; DNA damage; Chromosome segregation; Translocations; Genetic.
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