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The effects of myricitrin and vitamin E against reproductive changes induced by D-galactose as an aging model in female mice: An experimental study
Omidi, Mina; Ahangarpour, Akram; Mard, Seyed Ali & Khorsandi, Layasadat
Abstract
Background: Aging is accompanied by decreasing general function in the cells and
tissues. D-galactose (D-gal) induces aging and plays a role in the pathogenesis of it.
Myricitrin is a plant-derived antioxidant.
Objective: The present study was performed to evaluate the effects of myricitrin
on antioxidant defense, sex hormone levels, uterus, and ovarian histology in D-gal-induced
aging female mouse model.
Materials and Methods: In this experimental study, 72 female adult NMRI mice,
weighing 30-35 gr, 3-4 months old, were randomly divided into six groups (n = 12/each):
(I) Control (vehicle; normal saline), (II) D-gal at 500 mg/kg/d for 45 days, (III-V) D-gal +
myricitrin-treated groups (these groups received myricitrin at 5, 10, and 20 mg/kg/d,
and (VI) D-gal + 100 mg/kg/d vitamin E orally for the last 28 days. The antioxidant
indices were done on the basis of colorimetric method, and sex hormone levels were
measured by using enzyme-linked immunosorbent assay kits. Histological assessment
of the uterus and ovaries were also evaluated.
Results: D-gal impaired the estrous cycle, also degenerative changes occur in the
ovarian follicles and damage to the uterus and ovarian tissue occurs. In D-gal group,
the level of sex hormones (p = 0.03) and the total antioxidant capacity (p = 0.002)
decreased, while the level of malondialdehyde and gonadotropins increased (p = 0.03).
Myricitrin at lower doses and vitamin E ameliorated the D-gal effects.
Conclusion: These findings suggest that myricitrin at low doses can effectively prevent
D-gal-induced oxidation and aging in mice. The effect of myricitrin was equivalent and
sometimes better than vitamin E.
Keywords
Aging; D-galactose; Mice; Myricitrin; Vitamin E.
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