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Effect of monosodium glutamate on testicular tissue of paclitaxel-treated mice: An experimental study
Kianifard, Davoud; Ehsani, Ali; Daneshgar, Parisa Zeinolabedini; Akbari, Ghasem & Shoar, Seyyed Maysam Mousavi
Abstract
Background: Paclitaxel (PTX), a chemotherapeutic agent, and monosodium glutamate
(MSG) have oxidative effects on testicular tissue.
Objective: In this study, the effects of MSG administration on the exacerbation of
testicular tissue alterations related to PTX treatment were evaluated.
Materials and Methods: MSG (30 & 60 mg/kg i.p.) was administrated to six groups
(n = 8/each) of adult mice before or after PTX treatment: control, PTX-treated, MSG30
+ PTX, MSG60 + PTX, PTX + MSG30, and PTX + MSG60. Following the euthanizing,
the body weight measurement, pituitary–testicular axis hormonal analysis and serum
lipid peroxidation index assessment was prepared, testicular histomorphometry
(tubular diameter and germinal epithelium height), immunohistochemistry of p53
was completed. Microscopic indices of spermatogenesis (tubular differentiation,
spermiogenesis and repopulation indices) were studied.
Results: Body weight was not changed significantly. The levels of testosterone (p =
0.0001), follicle stimulating hormone (p = 0.019), and luteinizing hormone (p = 0.08) were
decreased while the level of lipid peroxidation index was increased (p = 0.208) in the
treated groups. The histomorphometry indices (p < 0.0001 and p = 0.001, respectively),
germ cells population (p < 0.05) and microscopic indices of spermatogenesis (p = 0.001,
p = 0.005, p < 0.0001, respectively) were significantly reduced in all treated groups. The
administration of MSG before PTX treatment induces more changes. The most positive
reaction to p53 was observed in MSG30 or 60 + PTX groups compared to other groups.
Conclusion: The administration of MSG could intensify testicular tissue alterations
related to PTX chemotherapy.
Keywords
Mice; Monosodium glutamate; Morphometry; Paclitaxel; Testicular tissue.
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