Evaluating the protective effect of resveratrol, Q10, and alpha-lipoic acid on radiation-induced mice spermatogenesis injury: A histopathological study|
Najafi, Masoud; Cheki, Mohsen; Amini, Peyman; Javadi, Abdolreza; Shabeeb, Dheyauldeen & Musa, Ahmed Eleojo
Background: Testis is one of the most sensitive organs against the toxic effect of ionizing
radiation. Exposure to even a low dose of radiation during radiotherapy, diagnostic radiology,
or a radiological event could pose a threat to spermatogenesis. This may lead to temporary or
permanent infertility or even transfer of genomic instability to the next generations.
Objective: In this study, we evaluated the protective effect of treatment with three natural antioxidants;
resveratrol, alpha lipoic acid, and coenzyme Q10 on radiation-induced spermatogenesis
Materials and Methods: 30 NMRI mice (6-8 wk, 30 ± 5 gr) were randomly divided into six
groups (n = 5/each) as 1) control; 2) radiation; 3) radiation + resveratrol; 4) radiation + alpha lipoic
acid; 5) radiation + resveratrol + alpha lipoic acid; and 6) radiation+ Q10. Mice were treated
with 100 mg/kg resveratrol or 200 mg/kg alpha lipoic acid or a combination of these drugs.
Also, Q10 was administered at 200 mg/kg. All treatments were performed daily from two days
before to 30 min before irradiation. Afterward, mice were exposed to 2 Gy 60Co gamma rays; 37
days after irradiation, the testicular samples were collected and evaluated for histopathological
Results: Results showed that these agents are able to alleviate some toxicological parameters
such as basal lamina and epididymis decreased sperm density. Also, all agents were able to
increase Johnsen score. However, they could not protect against radiation-induced edema,
atrophy of seminiferous tubules, and hyperplasia in Leydig cells.
Conclusion: This study indicates that resveratrol, alpha-lipoic acid, and Q10 have the potential
to reduce some of the side effects of radiation on mice spermatogenesis. However, they cannot
protect Leydig cells as a source of testosterone and seminiferous tubules as the location of
Radiation; Spermatogenesis; Resveratrol; Alpha-lipoic acid; Coenzyme Q10.