en |
Homing of adipose stem cells on the human amniotic membrane as a scaffold: A histological study
Zarei, Hooman; Karimpour, Abbasali; Khalatbary, Ali Reza & Amiri, Fereshteh Talebpour
Abstract
Background: The human amniotic membrane (HAM) is a suitable and effective scaffold
for cell culture and delivery, and adipose-derived stem cells (ADSCs) are an important
source of stem cells for transplantation and chondrogenic differentiation.
Objective: To assess the practicability of a cryopreserved HAM as a scaffold in cell
proliferation and differentiation in vitro.
Materials and Methods: In this experimental study, adipose tissue samples were
harvested from the inguinal region of male patients aged 15-30 years. Flow cytometry
was used to identify CD31, CD45, CD90, and CD105 markers in adipose stem
cells. HAM was harvested from donor placenta after cesarean section, washed,
trypsin-based decellularized trypsinized decellularized, and used as a scaffold via
three methods: 1) ADSCs were differentiated into chondrocytes on cell culture flasks
(monolayer method), and after 14 days of culture, the cells were transferred and cultured
on both sides of the HAM; 2) ADSCs were cultured and differentiated directly on both
sides of the HAM for 14 days (scaffold-mediated differentiation); and 3) chondrocytes
were differentiated with micromass culture for 14 days, transferred on HAM, and tissue
slides were histologically analyzed qualitatively.
Results: Flow cytometry confirmed the presence of mesenchymal stem cells.
Histological findings revealed that the cells adhered and grew well on the stromal layer
of HAM. Among the three methods, scaffold-mediated differentiation of ADSCs showed
the best results.
Conclusion: ADSCs have excellent attachment, viability, and differentiation capacity in
the stromal side of HAM. Additionally, the direct culture and differentiation of ADSCs
on HAM is more suitable than the culture of differentiated cells on HAM.
Keywords
Amniotic membrane; Scaffold; Chondrogenesis; Differentiation; Mesenchymal stem cell.
|