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International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
ISSN: 1680-6433
EISSN: 2008-2177
Vol. 18, No. 3, 2020, pp. 215-226
Bioline Code: rm20027
Full paper language: English
Document type: Research Article
Document available free of charge

International Journal of Reproductive BioMedicine, Vol. 18, No. 3, 2020, pp. 215-226

 en Low dose bitter leaf improves sperm quality disrupted in immunosuppressed Wistar rats: An experimental study
Kadir, Risikat Eniola; Ibrahim, Abdulmumin; Ibrahim, Balkis Abimbola; Gwadabe, Sadiya Musa; Jaji-Sulaimon, Rukayat; Adigun, Munirat Foyeke & Oyewopo, Adeoye Oyetunji


Background: Synthetic prednisolone (PRED) is a widely used over-the-counter glucocorticoid. Glucocorticoids have inhibitory effects on the immune system and are often used as immunosuppressive agents. Suppressed immunity may impact fertility via the hypothalamic-pituitary-adrenal axis. Bitter leaf (BL) has been shown to improve sperm parameters, but its effects on immunosuppression-associated infertility have not yet been documented.
Objective: To determine the fertility effects of bitter leaf on immunosuppressed Wistar rats.
Materials and Methods: A total of 30 male adult Wistar rats were randomly assigned to 6 groups (n = 5/each). Group A served as a control and were given distilled water in addition to normal feeds, group B received 2 mg/kg PRED for 14 days and served as the standard immunosuppressed group, and groups C-F were immunosuppressed as in B but in addition received 50 mg/kg levamisole, low-dose (250 mg/kg) BL, highdose (375 mg/kg) BL, and low-dose BL + levamisole, respectively. The CD4 counts, hematological parameters, and sperm parameters were analyzed and compared.
Results: There were significant decreases in sperm motility, progressive motility, morphology, and life/death ratio in the animals given PRED only compared to the controls (p = 0.002, 0.001, 0.001, and 0.01, respectively). These were significantly increased in the treated groups, and animals given levamisole and 250 mg/kg BL showed significantly increased sperm counts compared to the controls (p = 0.04 and p = 0.04, respectively).
Conclusion: Low-dose BL (250 mg/kg) restored the sperm parameters altered by prednisolone administration.

Bitter leaf; Immunosuppression; Infertility; Prednisolone; Rats.

© Copyright 2020 - Kadir et al.
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