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Comparing four laboratory three-parent techniques to construct human aged non-surrounded nucleolus germinal vesicle oocytes: A case-control study
Darbandi, Sara; Darbandi, Mahsa; Agarwal, Ashok; Khorram khorshid, Hamid Reza; Sadeghi, Mohammad Reza; Esteves, Sandro C.; Sengupta, Pallav; Dutta, Sulagna; Fathi, Zohreh; Zeraati, Hojjat & Akhondi, Mohammad Mehdi
Abstract
Background: The three-parent assisted reproductive technique may increase oocyte
competence.
Objective: In this case-control study, the suitability of germinal vesicle transfer
(GVT), synchronous ooplasmic transfer (sOT), asynchronous ooplasmic transfer using
cryopreserved MII oocyte (caOT), and asynchronous ooplasmic transfer using waste MII
oocyte (waOT) for maturation of the human-aged non-surrounded nucleolus germinal
vesicle-stage (NSN-GV) oocyte were investigated.
Materials and Methods: NSN-GV oocytes were subjected to four methods: group A
(GVT), B (sOT), C (caOT) D (waOT), and E (Control). The fusion rates, MI, MII, ICSI
observations and cleavage at 2-cell, 4-cell, and 8-cell stages were compared in the
groups.
Results: In GVT, none of the oocytes fused. In sOT, all oocytes fused, 20 achieved
the MI, 14 progressed to MII, 8 fertilized, 6 cleaved and 5, 4, and 3 achieved the 2-cells, 4-cells and 8-cells, respectively. In caOT, all oocytes fused and achieved the MI,
8 progressed to MII and fertilized, 6 cleaved and 6, 5, and 5 achieved the 2-cells, 4-cells, and 8-cells respectively. In waOT, all oocytes fused, 5 and 3 progressed to MI and
MII, respectively, but only one fertilized, cleaved and reached a 4-cells stage. In group
E, 6 and 2 oocytes progressed to MI and MII, respectively, and only one fertilized but
arrested at the zygote stage. caOT had the highest survival rate when compared to
sOT (p = 0.04), waOT (p = 0.002), and control (p = 0.001).
Conclusion: The caOT method was beneficial over sOT, waOT, and GVT in
supplementing the developmental capacity of human-aged NSN-GV oocytes.
Keywords
Assisted reproductive techniques; In vitro oocyte maturation techniques; Nuclear transfer techniques; Oocytes; Oocyte donation.
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