|
International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
ISSN: 1680-6433 EISSN: 1680-6433
Vol. 18, No. 8, 2020, pp. 597-604
|
Bioline Code: rm20064
Full paper language: English
Document type: Research Article
Document available free of charge
|
|
International Journal of Reproductive BioMedicine, Vol. 18, No. 8, 2020, pp. 597-604
en |
Effect of experimental hyperthyroidism on CatSper1 and CatSper2 genes expression in the seminiferous tubules of BALB/c mice: An experimental study
Sadeghi, Saeed; Jalali, Mahdi; Nikravesh, Mohamad Reza & Sankian, Mojtaba
Abstract
Background: CATSPER 1 (Cation Channel Sperm Associated 1) and CATSPER2
channels have an important role in sperm motility. In this study, the effects of
hyperthyroidism on Catsper1 and 2 genes of seminiferous tubules in mice testes were
investigated.
Objective: The present study was conducted to investigate the effect of
hyperthyroidism on the expression of CATSPER1 and CATSPER2 genes in the
seminiferous tubules of mice.
Materials and Methods: This study was conducted on 20 BALB/C male mice
divided into two groups - experimental and control. The experimental group was
administered with 500 mg/l levothyroxine (L-thyroxine) liquid solution for two months
for inducing hyperthyroidism, which was confirmed by radioimmunoassay. On the
other hand, the control group was kept in animal houses under a normal condition.
The implementation of real-time polymerase chain reaction and immunohistochemical
studies was accomplished after the removal of the testes of the mice under anesthesia
induced by chloroform.
Results: Results showed that there was no significant difference in CATSPER1 (p = 0.45)
and CATSPER2 (p = 0.34) gene expression between groups. At the same time, the color
intensity showed no significant enhancement in the hyperthyroidism group (CATSPER1
p = 0.17 and CATSPER2 p = 0.22) as compared to the control group.
Conclusion: Considering the key role of CATSPER in the molecular structure of the
sperm, our findings showed that the hyperactivity of the thyroid gland has no significant
effects on the function of these components. Therefore, it might be concluded that
hyperthyroidism has no considerable effects on the seminiferous tubules.
Keywords
CATSPER1; CATSPER2; Hyperthyroidism; Mice; Sperm.
|
|
© Copyright 2020 - International Journal of Reproductive BioMedicine Alternative site location: http://www.ijrm.ir
|
|