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International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
ISSN: 1680-6433 EISSN: 1680-6433
Vol. 18, No. 10, 2020, pp. 825-836
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Bioline Code: rm20083
Full paper language: English
Document type: Research Article
Document available free of charge
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International Journal of Reproductive BioMedicine, Vol. 18, No. 10, 2020, pp. 825-836
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Investigating the expressions of miRNA-125b and TP53 in endometriosis. Does it underlie cancer-like features of endometriosis? A case-control study
Hajimaqsoudi, Elnaz; Darbeheshti, Farzaneh; Kalantar, Seyed Mehdi; Javaheri, Atiyeh; Mirabutalebi, Seyed Hamidreza & Sheikhha, Mohammad Hasan
Abstract
Background: Endometriosis is generally considered as a benign condition; however, there is
a possibility for it to become cancerous. miR-125b is upregulated in both endometriotic tissues
and serum samples of women with endometriosis but its potential targets in endometriosis
are still not fully understood.
Objective: The role of miR-125b in the regulation of TP53 expression in endometriosis was
tested with a bioinformatics approach. In addition, the expression of miR-125b and TP53 in
both eutopic (Eu-p) and ectopic endometrium (Ec-p) in the endometrium tissues of women
with endometriosis was compared to those in the normal endometrium tissues of controls
(Normal).
Materials and Methods: In this case-control study, the Eu-p and Ec-p samples were collected
from 20 women who underwent laparoscopic surgery, and the normal endometrium tissues
were collected from 20 controls with no evidence of endometriosis. For bioinformatics
approach, a protein-protein interaction network was constructed based on co-expressed
potential targets of miR-125b. Quantitative polymerase chain reaction technique was used
for the measurement of miR125b and TP53 expression.
Results: Our results showed that miR-125b was significantly overexpressed in Ec-p (p-value:
0.021). In addition, there was a significant TP53 under expression in both the Ec-p and Eu-p
samples compared with the Normal tissues (p-value: 0.003).
Conclusion: The negative correlation between miR-125b and TP53 as well as a noticeable
decreased expression of TP53 in both Ec-p and Eu-p samples may be interpreted as the
roles of miR-125b/TP53 axis in the pathogenesis of endometriosis. In addition, these findings
and bioinformatic analyses imply a possible role of miR-125b in cancer-like features of
endometriosis.
Keywords
Endometriosis; TP53; miR-125b; Ectopic endometrium; Eutopic endometrium.
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© Copyright 2020 - Hajimaqsoudi et al. Alternative site location: http://www.ijrm.ir
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