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International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
ISSN: 1680-6433 EISSN: 1680-6433
Vol. 18, No. 10, 2020, pp. 855-864
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Bioline Code: rm20086
Full paper language: English
Document type: Research Article
Document available free of charge
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International Journal of Reproductive BioMedicine, Vol. 18, No. 10, 2020, pp. 855-864
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The effect of acrylamide on mitochondrial membrane potential and glutathione extraction in human spermatozoa: A laboratory study
Omidi, Zeinab; Piravar, Zeinab & Ramezani, Mina
Abstract
Background: Acrylamide (AA) is a compound used in the industrial production of
polyacrylamide. AAs affects by creating oxidative stress. It produces reactive oxygen
species and leads to lipid peroxide. Lipid peroxidation in the cell membrane is one
of the most important oxidations in the sperm, which can disrupt the fluidity and
permeability of cell membranes and damage all cells.
Objective: To investigate the different concentrations of AA on human sperm
parameters based on the World Health Organization standard and its impact on
mitochondrial membrane potential and sperm glutathione levels.
Materials and Methods: In this laboratory study, we examined the different
concentrations of AA on human sperm parameters based on the World Health
Organization standard and its impact on mitochondrial membrane potential by flow
cytometry and sperm glutathione levels by ELISA assay.
Results: The results were reported as the mean fluorescence intensity of JC and the
index was observed to decrease following the effect of AA in mitochondrial membrane
potential (Δ Ψm). The results of ELISA test to study the level of intracellular glutathione
showed that with the increase in the concentration of AA exposed to sperms, there
was a significant reduction in the level of intracellular glutathione.
Conclusion: AA destroys the sperm membrane integrity under apoptotic and oxidative
inductions with a negative impact on mitochondrial function and antioxidative enzyme
in sperm such as glutathione.
Keywords
Acrylamides; Spermatozoa; Mitochondrial membrane potential; Glutathione S-Transferase.
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