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Protective effects of hesperetin on the quality of sperm, apoptosis, lipid peroxidation, and oxidative stress during the process of cryopreservation: An experimental study
Valipour, Jamal; Mojaverrostami, Sina; Abouhamzeh, Beheshteh & Abdollahi, Masoumeh
Abstract
Background: Hesperetin is a bioflavonoid compound, largely used in Chinese
traditional medicine and found plenty in citrus fruits. Hesperetin has beneficial
effects against different diseases. The sperm cryopreservation process is a common
method that is used in infertility laboratories. It has been reported that during the
cryopreservation process, the quality of sperm is significantly reduced.
Objective: To investigate the effect of hesperetin on the quality of human spermatozoa
during the cryopreservation process.
Materials and Methods: In this experimental study, 22 sperm sample of
normozoospermia men who reffered to the infertility department of the Shariati
Hospital (Tehran, Iran) Between October and November 2019 were collect and divided
in to three groups as: 1) fresh, 2) control (frozen-thawed group without treatment), and
3) treatment group as frozen-thawed samples supplemented with 20 μM hesperetin.
Motility, Viability, morphology, Apoptotic-like changes, intracellular H2O2, intracellular
O2−, and lipid peroxidation (LPO) was measured.
Results: Hesperetin treatment during the cryopreservation process of human sperm
significantly improved the viability, motility, and morphology rates of the spermatozoa
after frozen-thawed process in control group (p < 0.01). In addition, it significantly
reduced the reactive oxygen species (ROS) level, LPO level and increased the
percentage of viable sperm cells with intact plasma membrane (p < 0.01) after frozen-thawed
process.
Conclusion: Hesperetin can improve the quality of human sperm and also protect
human sperm against reactive oxygen species, LPO, and apoptosis during the
cryopreservation-thawing process.
Keywords
Cryopreservation; Hesperetin; Spermatozoa; Reactive oxygen species.
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