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Reversible inhibition of sperm under guidance as an intratubular and reversible contraception in female rats: An experimental study
Ansari, Abdul Salam; Sevliya, Kiran; Badar, Ayesha & Lohiya, Nirmal Kumar
Abstract
Background: Reversible inhibition of sperm under guidance (“RISUG®”) is a promising
intravasal male contraceptive.
Objective: An exploratory study was conducted with a concept of non-invasive,
transcervical, single-intervention and reversible contraception using RISUG® in
females.
Materials and Methods: In this experimental study, 60 adult Wistar albino female rats
weighing 150-155 g, 3-4 months old were divided into four groups: group I: sham-operated
control; group II: tubal occlusion with RISUG for 90 days; group III: tubal
occlusion with RISUG® for 90 days and reversal with dimethyl sulphoxide and group IV:
tubal occlusion with RISUG® for 90 days and reversal with 5% NaHCO3. Animals were
subjected to bilateral fallopian tube occlusion with RISUG® and reversal with DMSO and
NaHCO3. The estrous cycle, fertility and histology of fallopian tube were evaluated.
Results: Group I showed 100% fertility during all mating schedules. Animals of
experimental groups indicated positive mating, but 0% fertility was evident following
30, 60, and 90 days of tubal occlusion. However, after reversal, fertility steadily
increased to normalcy in groups III (50% at 45 days, 80% at 105 days, 100% at 150 and
195 days) and IV (70% at 45 and 105 days, 100% at 150 and 195 days) animals. Group
II illustrated disorganized inner cell linings and eosinated RISUG® implant-filled lumen.
Reversal groups (III and IV) revealed complete restoration of cellular histo-architecture.
Regular estrous cycle was noticed in all experimental groups.
Conclusion: RISUG® is suitable for single intervention, intratubular, reversible
contraception in female rats.
Keywords
Contraception; Fallopian tube; Tubal occlusion; RISUG®; Reversible.
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