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Chronic stress increases the tyrosine phosphorylation in female reproductive organs: An experimental study
Bunsueb, Sudtida; Lapyuneyong, Natthapol; Tongpan, Saranya; Arun, Supatcharee & Iamsaard, Sitthichai
Abstract
Background: Changes in tyrosine-phosphorylated (TyrPho) protein expressions have
demonstrated stress in males. In females, chronic stress (CS) is a major cause of
infertility, especially anovulation. However, the tyrosine phosphorylation in the female
reproductive system under stress conditions has never been reported.
Objective: To investigate the alteration of TyrPho protein expression in ovary, oviduct,
and uterus of CS rats.
Materials and Methods: In this experimental study, 16 female Sprague-Dawley rats (5
wk: 220-250 gr) were divided into control and CS groups (n = 8/group). Every day, the CS
animals were immobilized within a restraint cage and individually forced to swim in cold
water for 60 consecutive days. Following the stress induction, the ovary, oviduct, and
uterus of all rats were observed for their morphologies. The total protein profiles of all
tissues were revealed by sodium dodecyl sulphate polyacrylamide gel electrophoresis
(SDS-PAGE) before detecting TyrPho proteins using western blot. Intensity analysis was
used to compare the expression of proteins between groups.
Results: The results showed that the morphology and weights of ovary and oviduct in
the CS group were not different from control. In contrast, the CS significantly increased
the uterine weight as compared to control. Moreover, the expressions of TyrPho
proteins in the ovary (72, 43, and 28 kDas), oviduct (170, 55, and 43 kDas), and uterus
(55, 54, and 43 kDas) were increased in CS group as compared to those of control.
Conclusion: The increased expressions of TyrPho proteins in ovary, oviduct, and uterus
could be potential markers used to explain some machanisms of female infertility
caused from chronic stress.
Keywords
Ovary; Oviduct; Uterus; Phosphorylation.
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