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Comparison of three sperm selection methods for ICSI-DGC, Cumulus column, and incubation with supernatant product of adipose tissue-derived adult stem cells: An experimental study
Yazdanpanah, Zeynab; Nasrabadi, Mitra Heydari & Piravar, Zeynab
Abstract
Background: The examination of sperm parameters and sperm DNA integrity are
necessary for male fertility expression. These parameters can be affected by method
of sperm separation.
Objective: To measure the damage of each sperm separation method on the sperm
parameters and sperm DNA integrity.
Materials and Methods: In this experimental study, semen samples of 20 infertile
men with asthenoteratozoospermia (Infertility Research Center, Qom, Iran, 2017) were
processed in three ways: density gradient centrifugation (DGC), cumulus column, and
incubation with supernatant products of adipose tissue-derived adult stem cells (SPAS).
The results of sperm parameters and DNA fragmentation before and after the process
were statistically analyzed.
Results: The number of separated sperms by normal morphologies during the SPAS
and the cumulus column was significantly more than the corresponding population
in the DGC group. In addition, although all three methods have the same ability to
increase total sperm motility and the number of recovered sperms, in the field of
forwarding movement and DNA fragmentation, the SPAS method performed more
efficiently (p = 0.021).
Conclusion: Sperm parameters and DNA fragmentation in the SPAS group were
better than those in the DGC and cumulus column groups. Furthermore, it has been
shown that the sperm capacity was increased with the SPAS method. However, the
rearrangement of sperm chromatin by reducing the disulfide bridges and providing
the possibility of re-histone over capacity causes a significant reduction in DNA
fragmentation.
Keywords
Sperm; DNA fragmentation; Cumulus oophorus column; SPAS.
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