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Association between a marker of sperm DNA damage and sperm indices in infertile males in Benin City, Nigeria: A cross-sectional study
Yusuf, Ilyas & Emokpae, Mathias Abiodun
Abstract
Background: Studies have shown oxidative DNA damage is associated with male
infertility.
Objective: This study determines the levels of 8-hydroxy-2’-deoxyguanosine (8-OHdG)
and some markers of oxidative stress in seminal fluid of males investigated for infertility
and men of proven fertility in Benin City, Nigeria.
Materials and Methods: Semen samples produced by self or assisted masturbation
were analyzed by microscopic technique according to the World Health Organization
guidelines. Thereafter, samples were centrifuged and seminal fluid plasma separated
and stored at -20°C prior to assay for 8-OHdG and oxidative stress biomarkers. Based
on the sperm concentration/count, the overall samples were grouped into the following
categories: normospermia (n = 20), oligozoospermia (n = 30), and azoospermia (n = 20).
The control group comprised of 30 age-matched males of proven fertility. The seminal
fluid 8-OHdG, total antioxidant status, superoxide dismutase and malondialdehyde
(MDA) were assayed through ELISA and spectrophotometric methods, respectively.
Results: Seminal plasma level of 8-OHdG and MDA were significantly higher (p = 0.01)
in infertile subjects than controls. The mean levels of 8-OHdG and MDA in infertile
subjects were higher in azoospermia than oligospermia than normospermia and so,
was least in the normospermia. Conversely, the mean levels of total antioxidant status
and superoxide dismutase were significantly lower (p = 0.01) in infertile than fertile the
control male subjects with levels higher in normospermia than oligospermia and least
in azoospermia. Moreover, the seminal 8-OHdG correlated negatively with sperm count
(r = -0.359, p = 0.01), percent motility (r = -0.388, p = 0.04), and percent morphology (r =
-0.327, p = 0.02).
Conclusion: The assessment of sperm DNA damage in addition to routine seminal fluid
analysis may play an important role in specific diagnosis and management of male
infertility.
Keywords
Male; Semen; Antioxidants; Sperm count; DNA damage.
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