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Effects of monosodium glutamate on testicular structural and functional alterations induced by quinine therapy in rat: An experimental study
Kianifard, Davoud; Mousavi Shoar, Seyyed Maysam; Fallah Karkan, Morteza & Aly, Ahmed
Abstract
Background: Quinine (QU) as an anti-malarial drug induces alterations in testicular
tissue. Toxic effects of monosodium glutamate (MSG) on the male reproductive system
have been recognized.
Objective: To investigate the impact of MSG administration on the intensity of
gonadotoxicity of QU.
Materials and Methods: Sixty eight-wk old Wistar rats weighing 180-200 gr were
divided into six groups (n = 10/each): the first group as a control; the second and
third groups received low and high doses of MSG (2 & 4 gr/kg i.p.), respectively,
for 28 days; the fourth group received QU for seven days (25 mg/kg); and in
the fifth and sixth groups, QU was gavaged following the MSG administration
(MSG + QU) from day 22 to day 28. Serum testosterone and malondialdehyde
(MDA) levels were measured. Testes samples were prepared for tissue MDA levels,
histomorphometry, and immunohistochemistry of p53. Sperm analysis was performed
on cauda epididymis.
Results: Serum and tissue MDA levels were increased in treated groups compared
to the control group. This increment was higher in the MSG + QU groups. The
testosterone levels were reduced significantly (p < 0.0001) in all treated groups. In
addition, histomorphometric indices and tubular epithelium population were reduced
significantly (p < 0.0001) in QU, MSG + QU, and consequently in high-dose MSG, QU,
MSG + QU groups. All spermatogenic indices were reduced in the treated groups,
particularly in the MSG + QU groups. Sperm motility and viability indices were reduced
significantly (p = 0.003) in the MSG + QU groups. Finally, the overexpression of p53
was observed in the MSG + QU groups.
Conclusion: The administration of MSG before and during QU therapy may intensify
testicular tissue alterations.
Keywords
Male reproductive system; Monosodium glutamate; Quinine hydrochloride; Rat.
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