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International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
ISSN: 1680-6433 EISSN: 1680-6433
Vol. 19, No. 4, 2021, pp. 361-370
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Bioline Code: rm21034
Full paper language: English
Document type: Research Article
Document available free of charge
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International Journal of Reproductive BioMedicine, Vol. 19, No. 4, 2021, pp. 361-370
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Follicular development and the expression of BAX and vascular endothelial growth factor in transplanted ovaries in uni- and bilateral ovariectomized mice: An experimental study
Dehghan, Maryam; Shahbazi, Shirin & Salehnia, Mojdeh
Abstract
Background: Several conflicting results have been reported on the survival and
function of transplanted ovaries.
Objective: Evaluation of the follicular development and the expression of vascular
endothelial growth factor (VEGF) and Bcl-2-associated X protein (BAX) in ovaries
transplanted into uni- and bilaterally ovariectomized mice.
Materials and Methods: In this experimental study, 40 female NMRI mice (21-days-old,
12-15 gr) were ovariectomized uni- and bilaterally (n = 20/ group), while the 8-wk-old
mice were considered as intact control group (n = 6). 5 weeks after transplantation
at the proestrus stage, the morphology of recovered transplanted ovaries and the
proportion of follicles were studied at different developmental stages. The apoptosis
cell death by pro-apoptotic protein BAX and the expression of VEGF were evaluated
using immunohistochemistry.
Results: In the bilaterally ovariectomized mice, among the 455 counted normal follicles,
a lower rate of primordial and primary follicles and a higher rate of preantral and antral
follicles were observed (p = 0.002). However, the percentages of preantral and antral
follicles, and the corpus luteum were significantly lower in the intact control group
(among the 508 counted normal follicles in this group) compared to other transplanted
groups (p = 0.002). The number of BAX-positive cells in all groups was not significantly
different. The VEGF expression was prominent in vessels of the corpus luteum, and
also in the theca layer of large follicles of studied groups.
Conclusion: Early discharge of ovarian reserve was prominent in the bilaterally
ovariectomized group but the incidence of apoptotic cells and VEGF expression
as angiogenic factor did not differ in both ovariectomized mice. Thus, unilaterally
ovariectomy has less side effects on the ovarian reserve compared to bilateral
ovariectomy.
Keywords
Autotransplantation; BAX protein; Vascular endothelial growth factor; Ovariectomy; Mice.
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© Copyright 2021 - Dehghan et al. Alternative site location: http://www.ijrm.ir
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