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International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
ISSN: 1680-6433 EISSN: 1680-6433
Vol. 19, No. 4, 2021, pp. 371-380
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Bioline Code: rm21035
Full paper language: English
Document type: Research Article
Document available free of charge
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International Journal of Reproductive BioMedicine, Vol. 19, No. 4, 2021, pp. 371-380
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Bovine oocyte developmental competence and gene expression following co-culturing with ampullary cells: An experimental study
Azari, Mehdi; Kafi, Mojtaba; Asaadi, Anise; Pakniat, Zohreh & Abouhamzeh, Beheshteh
Abstract
Background: There is no sufficient information on the impact of bovine ampullary
oviductal epithelial cells (BAOECs) on in vitro oocyte maturation competence and gene
expression.
Objective: This study aimed to examine the oocyte developmental competence
following co-culturing with a monolayer of fresh and frozen-thawed ampullary cells.
Materials and Methods: Bovine cumulus-oocyte complexes (COCs) were distributed
into three groups: control group; where in COCs were cultured in cell-free media for 24
hr and FML and FTML groups in which the COCs were cultured in maturation media for
18 hr and then transferred into a media containing fresh and frozen-thawed BAOECs
monolayer, respectively (BAOECs were extracted from the oviducts of slaughtered
cattle and were then cultured freshly or frozen-thawed) for a further 6 hr. After 24 hr,
the expanded COCs were evaluated for nuclear maturation, fertilization rate, and gene
expression (GDF9, StAR, CASP3, and FSHr).
Results: Nuclear maturation rate in the FTML group was significantly higher than the
control group (p = 0.02). The fertilization rate of FTML group was significantly higher
than the control and FML groups (p = 0.05 and p = 0.03, respectively). In terms of gene
expression, GDF9 were upregulated in the presence of the BAOECs during the last 6
hr of the in vitro maturation (p < 0.001). Furthermore, the expression of the StAR gene
in the FTML group was higher than the other groups (p = 0.02).
Conclusion: Ampullary cells co-culturing (especially frozen-thawed cells) for in vitro
maturation of bovine oocytes yields encourages the results and demonstrates the
beneficial effect of co-culture on gene expression and developmental competence.
Keywords
Ampulla; Bovine; Fertilization; Gene expression; IVM.
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