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International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
ISSN: 1680-6433 EISSN: 1680-6433
Vol. 19, No. 6, 2021, pp. 525-536
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Bioline Code: rm21052
Full paper language: English
Document type: Research Article
Document available free of charge
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International Journal of Reproductive BioMedicine, Vol. 19, No. 6, 2021, pp. 525-536
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Protective effect of vitamin E on sperm parameters, chromatin quality, and DNA fragmentation in mice treated with different doses of ethanol: An experimental study
Doostabadi, Mohamad Reza; Hassanzadeh-taheri, Mohammadmehdi; Asgharzadeh, Mahmoud & Mohammadzadeh, Masoomeh
Abstract
Background: Excessive consumption of alcohol induces an increase in oxidative stress
production and can lead to detrimental effects on the male reproductive system.
Objective: To evaluate the possible protective effects of coadministration of vitamin
(vit) E on the detrimental changes in the sperm quality of mice administered ethanol.
Materials and Methods: Fifty-four BALB/c mice were categorized into nine groups
(n = 6/each). The control group received a basal diet while the eight experimental
groups received ethanol 10%; ethanol 20%; vit. E 100 mg; vit. E 200 mg; ethanol 10%
+ vit. E 100 mg; ethanol 10% + vit. E 200 mg; ethanol 20% + vit. E 100 mg; ethanol
20% + vit. E 200 mg. After 35 days, the sperm parameters and sperm chromatin were
assessed.
Results: The results demonstrated a significant reduction in the motility rate, normal
morphology rate, viability rate, increase in abnormal DNA structure and packaging
(TB staining), and DNA damage (TUNEL) in ethanol consumer groups. In addition, the
findings showed a significant increase in the aforementioned parameters in ethanoland
vit. E-consumer groups compared to the ethanol-only consumer groups. The
ethanol group received 20% of the most damage among the groups. The group
receiving vit. E 100 mg and those receiving ethanol 10% + vit. E 200 mg gained the
highest benefit among the groups.
Conclusion: Sperm forward progressive motility, normal morphology rate, and viability
decreased in the ethanol groups. Also, the rates of spermatozoa with abnormal
DNA structure and DNA fragmentation increased in the ethanol groups. Our findings
revealed that the coadministration of vit. E and ethanol can protect destructive changes
in DNA structure and damage.
Keywords
Ethanol; Sperm parameters; Vitamin E.
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