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International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
ISSN: 1680-6433 EISSN: 1680-6433
Vol. 19, No. 6, 2021, pp. 559-568
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Bioline Code: rm21055
Full paper language: English
Document type: Research Article
Document available free of charge
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International Journal of Reproductive BioMedicine, Vol. 19, No. 6, 2021, pp. 559-568
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Association of M55L and Q192R polymorphisms of paraoxonase 1 gene (PON1) with recurrent pregnancy loss risk: A case–control study
Alizadeh, Mehdi; Nasiri, Mahboobeh; Samadi, Morteza; Ghasemi, Nasrin & Moradi, Ali
Abstract
Background: Recurrent pregnancy loss (RPL) refers to the incidence of two or more
abortions before the first half of pregnancy. Oxidative stress has been hypothesized to
play a central role in RPL.
Objective: To investigate the relationship between Q192R and L55M polymorphisms
of PON1 as antioxidant enzyme and the risk of RPL.
Materials and Methods: In this case–control study, 110 women with RPL (case) and 110
healthy fertile women (control) referred to the Research and Clinical Center for Infertility,
Shiraz, Iran were enrolled. Genomic DNA was extracted from the peripheral blood in all
participants. Polymorphisms were genotyped by polymerase chain reaction-restriction
fragment length polymorphism method.
Results: Statistical analysis of Q192R polymorphism showed a significant difference
for the RR genotype between the case and control group (OR = 11, CI = 1.39–86.87,
p = 0.005) but none for the QR and QQ genotypes. No significant association was
observed between the R and Q allelic frequency in the RPL participants compared to
the control group (p = 0.53). Also, statistical analysis of the L55M polymorphism for
MM genotype in the case group compared with the control group showed a significant
difference (OR = 3.59, CI = 0.97–13.30, p = 0.042), but none for the LM and LL genotypes.
Conclusion: The findings showed a significant correlation between the Q192R
polymorphisms and the L55M PON1 enzyme and RPL in this study population.
Keywords
Pregnancy; Abortion; PON1; Polymorphism; Recurrent pregnancy loss.
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