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The effect of follicular fluid selenium concentration on oocyte maturation in women with polycystic ovary syndrome undergoing in vitro fertilization/Intracytoplasmic sperm injection: A cross-sectional study
Poormoosavi, Seyedeh Mahsa; Behmanesh, Mohammad Amin; Varzi, Hossein Najafzadeh; Mansouri, Shabnam & Janati, Sima
Abstract
Background: A high level of free radicals and oxidative substances in women with
polycystic ovary syndrome (PCOS) can affect the ovaries through oxidative stress. Antioxidants such as selenium, a vital trace element in human health, can improve the
prognosis of PCOS by reducing oxidative stress.
Objective: This study was performed due to the lack of comprehensive information
about selenium concentration in follicular fluid and its effect on the oocyte count and
quality in infertile women with PCOS.
Materials and Methods: In this cross-sectional study, 78 women with PCOS
referred to Umm-al-Banin Infertility Clinic Center, Ganjavian Hospital, Dezful, Iran
for in-vitro fertilization from March to November 2019 were enrolled. After ovarian
stimulation with the antagonist protocol, the oocytes were retrieved under transvaginal
ultrasound in in-vitro fertilization/intracytoplasmic sperm injection cycles, and selenium
concentrations were measured in the follicular fluid using an atomic absorption method
by spectrophotometer device. Oocyte count and morphology were evaluated using
inverted optical microscopy.
Results: There were no significant differences between follicular fluid selenium
concentrations in terms of the total number of oocytes and immature oocytes in the
metaphase I and germinal vesicle stages. However, a significantly reduced number of
metaphase II oocytes was observed at selenium levels < 40 μg/dL (p = 0.001).
Conclusion: Based on our results, low levels of follicular selenium concentration in
infertile women with PCOS can reduce the quality and potency of oocyte maturation.
Keywords
Polycystic ovary syndrome; Oxidative stress; Selenium; In vitro fertilization; Oocyte quality; Follicular fluid.
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