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Detrimental effects of cerium oxide nanoparticles on testis, sperm parameters quality, and in vitro fertilization in mice: An experimental study
Hosseinalipour, Elnaz; Karimipour, Mojtaba & Ahmadi, Abbas
Abstract
Background: Cerium oxide nanoparticles (CeO2 NPs) as an important nanomaterial
have a wide range of applications in many fields and human beings’ exposure to this
nanomaterial is unavoidable. The effects of CeO2 NPs on the male reproductive system
are controversial.
Objective: To determine the effects of the administration of CeO2 NPs on the testis
tissue, sperm parameters, and in vitro fertilization (IVF) in mice.
Materials and Methods: Twenty-four male mice were divided into three groups
(n = 8/each): one control and two experimental groups receiving CeO2 NPs at doses of
50 and 100 mg/kg body weight, respectively, for 35 days. At the end of the experiment,
the diameter of seminiferous tubules (SNTs), epithelial height of SNTs, spermiogenesis
index in testes, sperm parameters (count, motility, viability, and morphology), sperm
chromatin condensation, DNA integrity, and IVF assays were analyzed.
Results: Histological results showed that the tubular diameter, the epithelial height
of the SNTs, and the spermiogenesis index were significantly decreased in the
experimental groups receiving CeO2 NPs. All sperm parameters in the experimental
groups were significantly reduced and, additionally, the percentages of immature
sperms and sperms with DNA damage were significantly increased in groups treated
with CeO2 NPs compared to the control. Furthermore, the rates of IVF and in vitro
embryo development were decreased.
Conclusion: Collectively, the current study showed that oral administration of CeO2
NPs in mice had detrimental effects on the male reproductive system through inducing
testicular tissue alterations, decreasing sperm parameters quality, and also diminishing
the IVF rate and in vitro embryonic development.
Keywords
Cerium oxide; Testis; Sperm; Fertilization; Mice.
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